Structure and genetic variation of Diplodia mutila on declining ashes (Fraxinus excelsior) in Poland

Wojciech Kraj , Tadeusz Kowalski , Marcin Zarek


This study encompassed 137 cultures of Diplodia mutila isolated from necrotic tissue of 3- to 20-year-old trees of Fraxinus excelsior with decline symptoms, originating from three geographic regions of Poland differing by climatic conditions. Fungus identification was conducted on the basis of morphological traits of cultures and confirmed by analysis of ribosomal DNA sequences. The structure and genetic variation of D. mutila populations was determined with the use of RAMS markers. Neighbor Joining analysis of ITS sequences of the examined isolates showed the greatest similarity to sequences of D. mutila isolated from F. excelsior and deposited in GenBank. Intra-population variation expressed by Dice coefficient and Shannon index was high and increased along with the shifting of a population’s location from mild climate (northern Poland) to more severe climate, characteristic for upland and mountainous areas (southern Poland). The mallest genetic distance was shown by pairs of populations close to one another, inside individual regions. AMOVA showed great share (91%) of genetic variation inside a population, whereas variation was very small between populations (6.4%) and regions (2.6%). Principal component analysis showed grouping of fungus populations in accordance with their geographic location.
Author Wojciech Kraj (FoF / DoFP)
Wojciech Kraj,,
- Department of Forest Pathology
, Tadeusz Kowalski (FoF / DoFP)
Tadeusz Kowalski,,
- Department of Forest Pathology
, Marcin Zarek (FoF / DoFP)
Marcin Zarek,,
- Department of Forest Pathology
Journal seriesJournal of Plant Pathology, ISSN 1125-4653, (A 20 pkt)
Issue year2013
No95 (3)
Keywords in EnglishDiplodia mutila, Fraxinus excelsior, ash decline, genetic variation, RAMS markers
ASJC Classification1110 Plant Science
Internal identifierWL/12/9
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.535; WoS Impact Factor: 2013 = 0.768 (2) - 2013=1.066 (5)
Citation count*
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?