The effect of parachlorophenylalanine treatment on the activity of gonadal and lactotrophic axes in native Polish crested chickens stimulated to broodiness

Małgorzata Gumułka , Anna Hrabia , Nataly Avital-Cohen , Krzysztof Andres , Israel Rozenboim

Abstract

Brooding behavior, a common characteristic of native breeds of the domestic chicken, is marked by elevated prolactin (PRL) levels, which is necessary for incubation and connected with changes in hypothalamic–pituitary–gonadal axis activity. Evidence indicates the serotoninergic system is a potent modulator of PRL secretion. The objective of this study is to investigate whether blocking serotonin synthesis with parachlorophenylalanine (PCPA) prevents incubation behavior in native Polish crested chickens. In addition, we examined the effect of PCPA on the gene expression of the gonadal and lactotrophic axes. Birds were stimulated to broodiness by artificial eggs in nests. At 34 wk of age (April: spring period), the hens were divided into 2 groups (14 hens in each group): control and PCPA-treated (50 mg/kg BW) group. After 5 wk of treatment, the artificial eggs were removed from the nests. Egg production, incubation activity, and levels of plasma ovarian steroids progesterone (P4), testosterone (T), estradiol (E2), and PRL were examined. At the end of the experiment (45 wk of age, June: summer period), ovarian characteristics and mRNA gene expression of gonadal (gonadotropin-releasing hormone [GnRH] I, luteinizing hormone [LH] ß, follicle-stimulating hormone [FSH] ß) and lactotrophic (vasoactive intestinal peptide [VIP], PRL) axes were measured by quantitative real-time PCR. Incubation activity was observed in the hens of both groups but with lower frequency in PCPA-treated birds. Moreover, the PCPA group had a higher cumulative egg production than the controls. During the first six and 8 wk of the experiment, levels of P4 and E2, respectively, were similar in both groups, but all concentrations increased in the PCPA-treated hens after this period. In addition, increased GnRH-I, LHß, and FSHß and decreased VIP mRNA expression was observed in the PCPA group compared with the controls. There were no differences in PRL mRNA expression, the PRL level, and ovarian morphometry between the 2 groups. These results indicate that blockage of serotonin synthesis by PCPA does not effectively prevent incubation in native Polish crested chickens. However, treatment with PCPA increased gonadal axis activity and improved reproductive performance.
Author Małgorzata Gumułka (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Małgorzata Gumułka,,
- Department of Animal Reproduction, Anatomy and Genomics
, Anna Hrabia (FoAS / Department of Animal Physiology and Endocrinology)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Nataly Avital-Cohen - The Hebrew University of Jerusalem
Nataly Avital-Cohen,,
-
, Krzysztof Andres (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Krzysztof Andres,,
- Department of Animal Reproduction, Anatomy and Genomics
, Israel Rozenboim - The Hebrew University of Jerusalem
Israel Rozenboim,,
-
Journal seriesPoultry Science, ISSN 0032-5791, e-ISSN 1525-3171, (N/A 140 pkt)
Issue year2020
Vol99
No5
Pages2708-2717
Publication size in sheets0.5
Keywords in Englishreproduction, native breed, incubation, lactotrophic axis, prolactin
ASJC Classification2700 General Medicine; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.psj.2019.12.015
URL https://www.sciencedirect.com/science/article/pii/S0032579119580609/pdfft?md5=570d4e7c27f87341db05b737f123c574&pid=1-s2.0-S0032579119580609-main.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Other open licence; after publication
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The effect of parachlorophenylalanine treatment on the activity of gonadal and lactotrophic axes in native Polish crested chickens stimulated to broodiness of 26-05-2020
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Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.38; WoS Impact Factor: 2019 = 2.659 (2) - 2019=3.103 (5)
Citation count*
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FinansowanieThis study wasfinancially supported by the Ministryof Science and Higher Education (SUB 215- D205) inPoland. The publication fee was covered by the Societyfor Biology of Reproduction in Poland
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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