The Impact of Photoperiod on the Leptin Sensitivity and Course of Inflammation in the Anterior Pituitary

Maciej Wójcik , Andrzej Przemysław Herman , Dorota Zięba-Przybylska , Agata Krawczyńska

Abstract

Leptin has a modulatory impact on the course of inflammation, affecting the expression of proinflammatory cytokines and their receptors. Pathophysiological leptin resistance identified in humans occurs typically in sheep during the long-day photoperiod. This study aimed to determine the effect of the photoperiod with relation to the leptin-modulating action on the expression of the proinflammatory cytokines and their receptors in the anterior pituitary under physiological or acute inflammation. Two in vivo experiments were conducted on 24 blackface sheep per experiment in different photoperiods. The real-time PCR analysis for the expression of the genes IL1B, IL1R1, IL1R2, IL6, IL6R, IL6ST, TNF, TNFR1, and TNFR2 was performed. Expression of all examined genes, except IL1β and IL1R2, was higher during short days. The leptin injection increased the expression of all examined genes during short days. In short days the synergistic effect of lipopolysaccharide and leptin increased the expression of IL1B, IL1R1, IL1R2, IL6, TNF, and TNFR2, and decreased expression of IL6ST. This mechanism was inhibited during long days for the expression of IL1R1, IL6, IL6ST, and TNFR1. The obtained results suggest the occurrence of leptin resistance during long days and suggest that leptin modulates the course of inflammation in a photoperiod-dependent manner in the anterior pituitary
Author Maciej Wójcik - Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Maciej Wójcik,,
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, Andrzej Przemysław Herman - Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Andrzej Przemysław Herman,,
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, Dorota Zięba-Przybylska (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Dorota Zięba-Przybylska,,
- Department of Nutrition, Animal Biotechnology and Fisheries
, Agata Krawczyńska
Agata Krawczyńska,,
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Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2020
Vol21
No11
Pages1-22
Publication size in sheets1.05
Article number4153
Keywords in EnglishLeptin; leptin resistance; IL1β; IL6; TNFα; pituitary; sheep; LPS; photoperiod; relative gene expression
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
DOIDOI:10.3390/ijms21114153
URL https://www.mdpi.com/1422-0067/21/11/4153/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Other open licence; after publication
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The Impact of Photoperiod on the Leptin Sensitivity and Course of Inflammation in the Anterior Pituitary of 15-06-2020
2,86 MB
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
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LicencjaOpen Access na stronie czasopisma 15.06.2020
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