The Viability of Serval (Leptailurus serval) and Pallas Cat (Felis manul) Oocytes after Cryopreservation Using the Rapid-I Method

Agnieszka Nowak , Joanna Kochan , Sylwia Prochowska , Agnieszka Partyka , Wiesława Młodawska , Wojciech Witarski , Józef Skotnicki , Teresa Grega , Marcin Pałys , Wojciech Niżański

Abstract

BACKGROUND: Vitrification by Rapid-I method could be essential for felid rescue programs to protect wild felid in the future. OBJECTIVE: This study was aimed at adapting the Rapid I method and evaluating the viability of serval and Pallas cat oocytes compared to oocytes of the domestic cat. MATERIALS AND METHODS: Oocytes after collection and in vitro maturation were vitrified using Cryotech medium (Cryotech, Japan) and a Rapid-I device (Vitrolife, Sweden). To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide. RESULTS: Survival rate in the control group (domestic cat) was 75 %. In the experimental group, 70% (serval) and 60% (pallas cat) viable oocytes were found. CONCLUSION: The Rapid-I method can be applied successfully for the vitrification of wild felid oocytes.
Author Agnieszka Nowak (FoAS / IoVS)
Agnieszka Nowak,,
- Institute of Veterinary Sciences
, Joanna Kochan (FoAS / IoVS)
Joanna Kochan,,
- Institute of Veterinary Sciences
, Sylwia Prochowska
Sylwia Prochowska,,
-
, Agnieszka Partyka
Agnieszka Partyka,,
-
, Wiesława Młodawska (FoAS / IoVS)
Wiesława Młodawska,,
- Institute of Veterinary Sciences
, Wojciech Witarski
Wojciech Witarski,,
-
, Józef Skotnicki
Józef Skotnicki,,
-
, Teresa Grega
Teresa Grega,,
-
, Marcin Pałys
Marcin Pałys,,
-
, Wojciech Niżański
Wojciech Niżański,,
-
Journal seriesCryoletters, [Cryo-Letters], ISSN 0143-2044, e-ISSN 1742-0644, (N/A 40 pkt)
Issue year2019
Vol40
No4
Pages226-230
Publication size in sheets0.5
Keywords in Englishwild felids, oocyte, Rapid-I, vitrification, bio-bank
ASJC Classification2700 General Medicine
URL http://www.cryoletters.org/Abstracts/vol_40_4_2019.htm#226
Languageen angielski
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.37; WoS Impact Factor: 2018 = 0.694 (2) - 2018=0.842 (5)
Citation count*
Additional fields
FinansowanieWe acknowledge the financial support from the National Centre for Research and Development grant NCBiR PBS3/B8/16/2015.
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