The Viability of Serval (Leptailurus serval) and Pallas Cat (Felis manul) Oocytes after Cryopreservation Using the Rapid-I Method
Agnieszka Nowak , Joanna Kochan , Sylwia Prochowska , Agnieszka Partyka , Wiesława Młodawska , Wojciech Witarski , Józef Skotnicki , Teresa Grega , Marcin Pałys , Wojciech Niżański
AbstractBACKGROUND: Vitrification by Rapid-I method could be essential for felid rescue programs to protect wild felid in the future. OBJECTIVE: This study was aimed at adapting the Rapid I method and evaluating the viability of serval and Pallas cat oocytes compared to oocytes of the domestic cat. MATERIALS AND METHODS: Oocytes after collection and in vitro maturation were vitrified using Cryotech medium (Cryotech, Japan) and a Rapid-I device (Vitrolife, Sweden). To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide. RESULTS: Survival rate in the control group (domestic cat) was 75 %. In the experimental group, 70% (serval) and 60% (pallas cat) viable oocytes were found. CONCLUSION: The Rapid-I method can be applied successfully for the vitrification of wild felid oocytes.
|Journal series||Cryoletters, [Cryo-Letters], ISSN 0143-2044, e-ISSN 1742-0644, (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||wild felids, oocyte, Rapid-I, vitrification, bio-bank|
|Publication indicators||= 0; : 2018 = 0.37; : 2018 = 0.694 (2) - 2018=0.842 (5)|
|Finansowanie||We acknowledge the financial support from the National Centre for Research and Development grant NCBiR PBS3/B8/16/2015.|
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