Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Magdalena Wójcik-Jagła , Marcin Rapacz , Ewa Dubas , Monika Krzewska , Przemysław Kopeć , Anna Nowicka , Agnieszka Ostrowska , Sabina Malaga , Iwona Żur

Abstract

Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.
Author Magdalena Wójcik-Jagła (FoAaE / DoPP)
Magdalena Wójcik-Jagła,,
- Department of Plant Physiology
, Marcin Rapacz (FoAaE / DoPP)
Marcin Rapacz,,
- Department of Plant Physiology
, Ewa Dubas
Ewa Dubas,,
-
, Monika Krzewska
Monika Krzewska,,
-
, Przemysław Kopeć
Przemysław Kopeć,,
-
, Anna Nowicka
Anna Nowicka,,
-
, Agnieszka Ostrowska
Agnieszka Ostrowska,,
-
, Sabina Malaga
Sabina Malaga,,
-
, Iwona Żur
Iwona Żur,,
-
Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2020
Vol21
No6
Pages1-17
Publication size in sheets0.8
Keywords in English barley; doubled haploid lines; freezing tolerance; drought tolerance; candidate genes; gene expression
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
DOIDOI:10.3390/ijms21062062
URL https://www.mdpi.com/1422-0067/21/6/2062
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
ijms-21-02062.pdf 1,23 MB
Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
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