Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley
Magdalena Wójcik-Jagła , Marcin Rapacz , Ewa Dubas , Monika Krzewska , Przemysław Kopeć , Anna Nowicka , Agnieszka Ostrowska , Sabina Malaga , Iwona Żur
AbstractPlant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)|
|Publication size in sheets||0.8|
|Keywords in English||barley; doubled haploid lines; freezing tolerance; drought tolerance; candidate genes; gene expression|
|ASJC Classification||; ; ; ; ; ; ;|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 0; : 2018 = 1.224; : 2018 = 4.183 (2) - 2018=4.331 (5)|
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