Changes in proteolytic activity and protein carbonylation in shoots of Alyssum montanum ecotypes under multi-metal stress

Ewa Muszyńska , Mateusz Labudda , Ewa Hanus-Fajerska

Abstract

The aim of present study was to evaluate the proteolytic response of metallicolous (M) and nonmetallicolous (NM) ecotypes of Alyssum montanum under heavy metals (HMs) stress. Therefore, shoot cultures of tested species grown on medium enriched simultaneously with 0.7 mM ZnSO4, 3.0 μM Pb(NO3)2 and 16.4 μM CdCl2 and these concentration corresponded to the content of their soluble forms marked in calamine substrate. After 8 weeks of cultivation, the overall protease activity (azocaseinolytic) and determination of the proteolytic (gelatinolytic) enzymes profile were estimated in HMs-treated and untreated specimens. The proteins of NM specimens were more susceptible to proteolysis induced by HMs than proteins of M ones. It was found that applied HMs ions caused an increase of protease activity in HMs-treated shoots of NM ecotype that was accompanied by diminished total soluble proteins content and their higher carbonylation. In contrast, the activities of the neutral proteases and metal-dependent serine proteases decreased in HMs-treated shoots of M ecotype. Our results have revealed significant differences at the protein metabolism level in contrasting A. montanum ecotypes cultured in vitro in the presence of HMs.
Author Ewa Muszyńska
Ewa Muszyńska,,
-
, Mateusz Labudda
Mateusz Labudda,,
-
, Ewa Hanus-Fajerska (FoBaH / IoPBaB / DoBaPP)
Ewa Hanus-Fajerska,,
- Department of Botany and Plant Physiology
Journal seriesJournal of Plant Physiology, ISSN 0176-1617, e-ISSN 1618-1328, (N/A 100 pkt)
Issue year2019
Vol232
Pages61-64
Publication size in sheets0.5
Keywords in EnglishHeavy metals, Metal-dependent serine protease, Protein oxidation, Proteolysis, Proteolytic enzyme
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science; 1314 Physiology
DOIDOI:10.1016/j.jplph.2018.11.013
URL https://www.sciencedirect.com/science/article/pii/S0176161718306448/pdfft?md5=ad739f1701e4ef226b38b5827a8d6a2a&pid=1-s2.0-S0176161718306448-main.pdf
Internal identifierWBIO/2019/11
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.174; WoS Impact Factor: 2018 = 2.825 (2) - 2018=3.236 (5)
Citation count*
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FinansowanieThis research was partially financed by Warsaw University of Life Sciences-SGGW, grant no 505-10-011100-P00136-99 awarded to EM.
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