Developmental changes in survivin mRNA expression in the chicken oviduct

Anna Hrabia , Agnieszka Leśniak , Andrzej Sechman

Abstract

Survivin is the smallest family member of the protein inhibitors of apoptosis. It acts as a bifunctional protein regulating both apoptotic cell death and cell proliferation. Survivin expression is largely limited to undifferentiated, mitotically active tissues. So far, there is no information regarding survivin expression and function in the oviduct of birds. Therefore, the purpose of the present study was to examine the expression of survivin mRNA in the chicken oviduct with respect to its pubertal development. Experiment was performed on Hy-Line Brown chickens (n=30) decapitated every second week from 10 to 16 weeks of age and at 17 weeks i.e at the time of sexual maturation. Four oviductal parts (the infundibulum, magnum, isthmus and shell gland) were isolated and survivin-142 mRNA expression was determined by quantitative real-time PCR. The oviductal weight [g] slightly elevated until 14 weeks of age (0.12 ± 0.01 → 2.22 ± 1.06) and sharply increased at 16 weeks (30.3 ± 6.78). At 17 weeks (maturation) the weight of the oviduct was 50.5 ± 4.29. The relative expression (RQ) of examined gene during sexual maturation in the infundibulum, magnum and shell gland gradually increased from 10 to 14 weeks of age, and next sharply decreased at 16 weeks followed by more pronounced reduction at 17 weeks. In the isthmus survivin mRNA expression did not change between 10 and 14 weeks and after that gradually declined. In conclusion, the results demonstrate the changes in expression of survivin in the oviduct parallel with the changes in oviductal weight and support the possibility that survivin is an important regulator of the chicken oviduct development.
Author Anna Hrabia (DoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Agnieszka Leśniak (DoAS / DoAPaE)
Agnieszka Leśniak,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (DoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Journal seriesReproductive Biology, ISSN 1642-431X, (A 15 pkt)
Issue year2013
Vol13
NoSupplement 1 : The 3rd Winter Workshop of the Society for Biology of Reproduction
Pages41-41
Publication size in sheets0.3
Conference3rd Winter Workshop of the Society for Biology of Reproduction: “Central and Local Regulations of Reproductive Processes”, Zakopane, 30-01-2013 - 01-02-2013, Polska
ASJC Classification1309 Developmental Biology; 1310 Endocrinology; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.repbio.2012.11.042
URL http://www.sciencedirect.com/science/article/pii/S1642431X12000800
Languageen angielski
Not used for evaluationyes
Score (nominal)0
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.63; WoS Impact Factor: 2013 = 1.048 (2) - 2013=1.479 (5)
Citation count*
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FinansowanieSupported by grant no. 2011/01/B/NZ4/03665 from National Science Centre, Poland
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