Storage of boar semen at 16-18 °C in the long-term commercial extender prepared with deionized water or nanowater

Joanna Szymanowicz , Tomasz Schwarz , Maciej Murawski , Martyna Małopolska , Zdzisław Oszczęda , Ryszard Tuz , Jacek Nowicki , Paweł Mieczysław Bartlewski


The aim of this study was to assess the usefulness of nanowater (NW; water declusterized using cold plasma treatment) as a diluent for a commercial boar semen extender during the 15-day storage (Days 1 to 15) at 16-18 °C. Ejaculates collected from 8 boars were subjected to the standard evaluation and then diluted in the extender prepared with deionized water (DW) or NW to a final concentration of 3×109 spermatozoa/ml. The proportion of defective spermatozoa increased (P<0.05) from Day 10 to Day 15 of storage (22.8±16.6% to 41.8±26.4% in DW group and 18.6±11.7% to 34.8±25.4% in NW group) and it was significantly greater in DW group compared with NW group on Days 5 and 10 due mainly to a greater (P<0.05) number of mid-piece defects in semen stored in the DW-containing extender. Sperm progressive motility decreased (P<0.05) in both groups between Days 2 and 6, Days 6 and 10, and Days 10 and 12, whereas the percentage of motile spermatozoa declined (P<0.05) to Day 14 only in NW group. Sperm motility was greater (P<0.05) in NW group compared with DW group from Day 5 to Day 13. A decline in sperm progressive motility below 40% in all semen samples occurred by Day 11 in DW group and by Day 12 in NW group. The mean survival time of sperm at 37 °C ex situ was greater in NW group than in DW group on Day 5 (314±87 min compared with 284±87 min) and Day 10 (223±34 min compared with 182±27 min; NW group compared with DW group, respectively). There were no differences (P>0.05) between the two groups in the concentrations of alkaline phosphatase and aspartate aminotransferase in semen extender. To summarize, the use of NW as an extender diluent exerts cytoprotective effects on boar spermatozoa and delays a decline in sperm progressive motility.
Author Joanna Szymanowicz (FoAS / DoAB)
Joanna Szymanowicz,,
- Department of Animal Biotechnology
, Tomasz Schwarz (FoAS / DoSaSRB)
Tomasz Schwarz,,
- Department of Swine and Small Ruminant Breeding
, Maciej Murawski (FoAS / DoAB)
Maciej Murawski,,
- Department of Animal Biotechnology
, Martyna Małopolska
Martyna Małopolska,,
, Zdzisław Oszczęda
Zdzisław Oszczęda,,
, Ryszard Tuz (FoAS / DoSaSRB)
Ryszard Tuz,,
- Department of Swine and Small Ruminant Breeding
, Jacek Nowicki (FoAS / DoSaSRB)
Jacek Nowicki,,
- Department of Swine and Small Ruminant Breeding
, Paweł Mieczysław Bartlewski
Paweł Mieczysław Bartlewski,,
Journal seriesAnimal Reproduction, ISSN 1806-9614, e-ISSN 1984-3143, (N/A 40 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in Englishpig, sperm, liquid storage, semen extender, nanowater
ASJC Classification3400 General Veterinary; 1103 Animal Science and Zoology
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
Storage of boar semen at 16-18 °C in the long-term commercial extender prepared with deionized water or nanowater of 05-12-2019
1,26 MB
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.4; WoS Impact Factor: 2018 = 0.985 (2) - 2018=1.125 (5)
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