In vitro Effects of TCDD, PCB126 and PCB153 on Estrogen Receptors, Caspases and Metalloproteinase-2 mRNA Expression in the Chicken Stell Gland

Anna Hrabia , Agnieszka Leśniak , Andrzej Sechman

Abstract

Among the environmental chemicals which disturb endocrine functions, dioxins and polychlorinated biphenyls (PCBs) are known as the most toxic. Numerous studies in mammals revealed that dioxins and PCBs disrupt functions of the uterus, delay implantation and increase embryo loss. The direct effect of these chemicals on the avian oviduct is not known. Therefore, in the study chicken shell gland tissues were used to examine the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), coplanar PCB126 and non-coplanar PCB153 on estrogen receptors (ERs), initiator caspase-1, executioner caspase-3 and metalloproteinase-2 (MMP-2) mRNA expression. Fragments of shell gland tissue isolated from the laying chicken were incubated for 24h with TCDD (100nM), PCB126 (100nM) or PCB153 (100 FM). Quantitative PCR analysis showed that: (1) TCDD increased ER beta (ERâ) mRNA expression, (2) PCB126 increased ER alpha (ERá), ERâ and caspase-1, and decreased MMP-2 mRNA expression, (3) PCB153 elevated the ERâ and caspase-1 expression levels and (4) expression of caspase-3 was not altered by any investigated xenobiotics. The results obtained using the shell gland explants model indicate that dioxins and PCBs have a direct effect on the chicken oviduct, especially the shell gland, by affecting the expression of genes involved in the function of this oviductal segment. It is suggested that coplanar PCBs such as PCB126, by changing cellular and extracellular regulators gene expression, may lead to disruption of shell gland activity and impair egg components formed in this organ.
Author Anna Hrabia (DoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Agnieszka Leśniak (DoAS / DoAPaE)
Agnieszka Leśniak,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (DoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Journal seriesFolia Biologica-Krakow, ISSN 0015-5497, (A 20 pkt)
Issue year2013
Vol61
No3-4
Pages277-282
Publication size in sheets0.5
Keywords in EnglishERs, caspases, MMP-2, TCDD, PCB, chicken shell gland
ASJC Classification1300 General Biochemistry, Genetics and Molecular Biology; 2700 General Medicine
DOIDOI:10.3409/fb61_3-4.277
URL http://www.isez.pan.krakow.pl/journals/folia/pdf/61(3-4)/61(3-4)_21.pdf
Internal identifierWHiBZ/2013/89
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 7; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.768; WoS Impact Factor: 2013 = 0.478 (2) - 2013=0.642 (5)
Citation count*
Additional fields
FinansowanieSupported by projects No. DS-3243/KFiEZ and N N303 561 339 (2010-2013) from the Ministry of Education and Science, Poland (to A.SECHMAN)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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