Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Agata Ptak , Anna El Tachny , Edyta Skrzypek , Tomasz Wójtowicz , Dominique Laurain-Mattar

Abstract

In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).
Author Agata Ptak (FoAE / DoPBaS)
Agata Ptak,,
- Department of Plant Breeding and Seed
, Anna El Tachny
Anna El Tachny,,
-
, Edyta Skrzypek
Edyta Skrzypek,,
-
, Tomasz Wójtowicz (FoAE / DoPBaS)
Tomasz Wójtowicz,,
- Department of Plant Breeding and Seed
, Dominique Laurain-Mattar
Dominique Laurain-Mattar,,
-
Journal seriesCentral European Journal of Biology, ISSN 1895-104X, [1644-3632 ], (A 20 pkt)
Issue year2013
Vol8
No6
Pages591-599
Publication size in sheets0.5
Keywords in EnglishSomatic embryos, 2,4-D, Dicamba, Picloram, Alkaloids, Phenolic compounds
ASJC Classification1100 General Agricultural and Biological Sciences; 2400 General Immunology and Microbiology; 1300 General Biochemistry, Genetics and Molecular Biology; 2800 General Neuroscience
DOIDOI:10.2478/s11535-013-0160-y
URL http://www.researchgate.net/publication/235961927_Influence_of_auxins_on_somatic_embryogenesis_and_alkaloid_accumulation_in_Leucojum_aestivum_callus
Internal identifierWRE/2013/21
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 14; WoS Impact Factor: 2013 = 0.633 (2) - 2013=0.748 (5)
Citation count*5 (2015-08-03)
Additional fields
FinansowanieDS/3129/KHRiN
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