Statistical evaluation of growth parameters in biofuel waste as a culture medium for improved production of single cell protein and amino acids by Yarrowia lipolytica

Monika Elżbieta Jach , Tomasz Bajda , Marek Juda , Robert Świder , Barbara Mickowska , Anna Malm


Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to be proficient in producing single cell protein, amino acids, and vitamin B12 while utilizing biofuel waste instead of a complete YPD medium for yeast growth. A fractional fractal design experiment was then applied, and the two fermentation parameters of temperature and pH were recognized to have a significant effect on the protein and amino acid production. Subsequently, the response surface methodology with a three-level complete factorial design was employed to optimize these influential parameters. Therefore, five different measuring systems were utilized to construct a quadratic model and a second-order polynomial equation. Optimal levels of parameters were then obtained by analysis of the model and the numerical optimization method. When the Y. lipolytica A-101 was cultivated at optimized pH (5.0) using biofuel waste as a medium, the protein concentration was increased to 8.28—a 44% enhancement as compared to the original (3.65). This study has thus demonstrated a beneficial way to cultivate Y. lipolytica A-101 on biofuel waste for enhanced production of single cell protein and amino acids for use in human diet and in animal feed.
Author Monika Elżbieta Jach - Katolicki Uniwersytet Lubelski Jana Pawła II w Lublinie (KUL)
Monika Elżbieta Jach,,
, Tomasz Bajda - Uniwersytet Medyczny w Lublinie
Tomasz Bajda,,
, Marek Juda - Uniwersytet Medyczny w Lublinie
Marek Juda,,
, Robert Świder - Katolicki Uniwersytet Lubelski Jana Pawła II w Lublinie (KUL)
Robert Świder,,
, Barbara Mickowska (FoFT / DoToCaC)
Barbara Mickowska,,
- Department of Technology of Catering and Consumption
, Anna Malm - Uniwersytet Medyczny w Lublinie
Anna Malm ,,
Journal seriesAMB Express, ISSN 2191-0855, (N/A 70 pkt)
Issue year2020
Publication size in sheets0.55
Keywords in EnglishAmino acids, Biofuel waste, Fermentation optimization, Single cell protein, Yarrowia lipolytica
ASJC Classification1304 Biophysics; 2402 Applied Microbiology and Biotechnology
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
s13568-020-00968-x.pdf 4,5 MB
Score (nominal)70
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.727; WoS Impact Factor: 2018 = 2.226 (2) - 2018=2.485 (5)
Citation count*
Additional fields
FinansowanieThis work was supported by the European Regional Development Fund under the Innovative Economy Operational Programme 2007-2013 (UDA-POIG-01.04.00-24-132/11-00).
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