Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs

Konrad Ocalewicz , Artur Gurgul , Klaudia Pawlina-Tyszko , Tomasz Szmatoła , Igor Jasielczuk , Monika Bugno-Poniewierska , Stefan Dobosz

Abstract

Ionizing radiation is administered to damage nuclear genome in fish eggs during induced androgenesis. In this study, we examined whether 350 Gy of X-ray applied to damage chromosomes in the rainbow trout eggs affects maternal RNA. Shortly after irradiation, we did not find any symptoms of RNA degradation in the treated eggs. Significant (p < 0.01) differences between non-irradiated and irradiated eggs concerned only a few transcripts including increased expression of immediate early response 2 (IER2) and early growth response 1 (EGR1) genes observed in the irradiated eggs. Both genes belong to the group of “immediate early genes” that respond quickly to the diverse extracellular stimuli. Elevated expression of these genes was accompanied by decreased level of ssa-miR-10b-5p and ssa-miR-21b-5p (p < 0.05), for which IER2 and EGR1 are target genes. The level of RNA in the fertilized irradiated eggs was highly significantly lower than in the non-irradiated eggs (p < 0.001) and in the unfertilized irradiated eggs (p < 0.0001). However, transcriptome profiles of fertilized non-irradiated eggs and fertilized irradiated eggs did not differ significantly. Thus, we assume that reduced abundance of mRNA in the fertilized irradiated eggs was associated with post-translational degradation and clearance of the maternal transcripts rather than from the irradiation of eggs.
Author Konrad Ocalewicz
Konrad Ocalewicz,,
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, Artur Gurgul
Artur Gurgul,,
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, Klaudia Pawlina-Tyszko
Klaudia Pawlina-Tyszko,,
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, Tomasz Szmatoła (VET)
Tomasz Szmatoła,,
- University Centre for Veterinary Medicine UJ-UR
, Igor Jasielczuk (VET)
Igor Jasielczuk ,,
- University Centre for Veterinary Medicine UJ-UR
, Monika Bugno-Poniewierska (FoAS / IoVS)
Monika Bugno-Poniewierska,,
- Institute of Veterinary Sciences
, Stefan Dobosz
Stefan Dobosz,,
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Journal seriesScientific Reports, ISSN 2045-2322, (N/A 140 pkt)
Issue year2019
No9
Pages1-12
Publication size in sheets0.55
Article number8084
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/s41598-019-44568-7
URL https://www.nature.com/articles/s41598-019-44568-7.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs of 06-08-2019
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Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2018 = 4.011 (2) - 2018=4.525 (5)
Citation count*
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OświadczenieT. Szmatoła, I. Jasielczuk - podwójna afiliacja
FinansowanieThis work was financed by the National Science Centre (NCN) (grant number 2014/15/B/ NZ9/00510).
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