Cryopreservation of Galanthus elwesii Hook. Apical meristems by droplet vitrification

Małgorzata Maślanka , B. Panis , Anna Bach

Abstract

The aim of this study was to develop an efficient cryopreservation protocol for the geophyte giant snowdrop (Galanthus elwesii Hook.) that guarantees a high rate of survival and plant regeneration after cryopreservation. The excised apical meristems were obtained from cultures of in vitro grown bulb scales. Using a vitrification procedure and optimizing the duration of the exposure to the loading solution (LS), meristem post-rewarm survival rates higher than 90 percent were achieved. Also regrowth percentages were very high, ranging from 87 to 91 percent. After optimizing the time of exposure to the plant vitrification solution (PVS2), the survival rate was between 83 and 97 percent. During post-rewarm regeneration, good growth recovery was as high as 76 percent; however, hyperhydration and callusing were also observed. The results demonstrate that cryopreservation of Galanthus elwesii germplasm seems to be feasible.
Author Małgorzata Maślanka (FoBaH / DoDP)
Małgorzata Maślanka,,
- Department of Decorative Plants
, B. Panis
B. Panis,,
-
, Anna Bach (FoBaH / DoDP)
Anna Bach,,
- Department of Decorative Plants
Journal seriesCryoletters, ISSN 0143-2044, (A 20 pkt)
Issue year2013
Vol34
No1
Pages1-9
Publication size in sheets0.5
Keywords in Englishcryopreservation, droplet vitrification, meristem, Galanthus elwesii
ASJC Classification2700 General Medicine
URL http://www.ncbi.nlm.nih.gov/pubmed/23435704
Internal identifierWBIO/2013/81
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.651; WoS Impact Factor: 2013 = 0.64 (2) - 2013=0.767 (5)
Citation count*8 (2015-11-11)
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