The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine

Małgorzata Pierzchalska , Małgorzata Panek , Małgorzata Czyrnek , Maja Grabacka

Abstract

The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2 to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
Author Małgorzata Pierzchalska (FoFT / DoFB)
Małgorzata Pierzchalska,,
- Department of Food Biotechnology
, Małgorzata Panek (FoFT / DoFB)
Małgorzata Panek,,
- Department of Food Biotechnology
, Małgorzata Czyrnek (FoFT / DoFB)
Małgorzata Czyrnek,,
- Department of Food Biotechnology
, Maja Grabacka (FoFT / DoFB)
Maja Grabacka,,
- Department of Food Biotechnology
Pages135-144
Publication size in sheets0.5
Book Turksen Kursad (eds.): Organoids : Stem Cells, Structure, and Function, Methods in Molecular Biology, 2019, Humana Press, ISBN 978-1-4939-7616-4, [978-1-4939-7617-1], DOI:10.1007/978-1-4939-7617-1
Keywords in EnglishIntestinal epithelium, Three-dimensional cell culture, Epithelial organoids, Chicken embryo, Intestinal myofibroblasts
DOIDOI:10.1007/7651_2016_15
URL https://link.springer.com/protocol/10.1007%2F7651_2016_15
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); after publication
Score (nominal)20
Score sourcepublisherList
Citation count*
Additional fields
FinansowanieThe authors acknowledge the financial support of Polish National Science Center (grant no. 2013/09/B/NZ9/00285 to M.P.)
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