The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
Małgorzata Pierzchalska , Małgorzata Panek , Małgorzata Czyrnek , Maja Grabacka
AbstractThe intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2 to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
|Publication size in sheets||0.5|
|Book||Turksen Kursad (eds.): Organoids : Stem Cells, Structure, and Function, Methods in Molecular Biology, 2019, Humana Press, ISBN 978-1-4939-7616-4, [978-1-4939-7617-1], DOI:10.1007/978-1-4939-7617-1|
|Keywords in English||Intestinal epithelium, Three-dimensional cell culture, Epithelial organoids, Chicken embryo, Intestinal myofibroblasts|
|License||Journal (articles only); author's original; ; after publication|
|Finansowanie||The authors acknowledge the financial support of Polish National Science Center (grant no. 2013/09/B/NZ9/00285 to M.P.)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.