Fully automated process for histamine detection based on magnetic separation and fluorescence detection

Milica Gagic , Lukas Nejdl , Kledi Xhaxhiu , Natalia Cernei , Ondrej Zitka , Ewelina Jamróz , Pavel Svec , Lukas Richtera , Pavel Kopel , Vedran Milosavljević , Vojtech Adam


To ensure food safety and to prevent unnecessary foodborne complications this study reports fast, fully automated process for histamine determination. This method is based on magnetic separation of histamine with magnetic particles and quantification by the fluorescence intensity change of MSA modified CdSe Quantum dots. Formation of Fe2O3 particles was followed by adsorption of TiO2 on their surface. Magnetism of developed probe enabled rapid histamine isolation prior to its fluorescence detection. Quantum dots (QDs) of approx. 3 nm were prepared via facile UV irradiation. The fluorescence intensity of CdSe QDs was enhanced upon mixing with magnetically separated histamine, in concentration-dependent manner, with a detection limit of 1.6 μM. The linear calibration curve ranged between 0.07 and 4.5 mM histamine with a low LOD and LOQ of 1.6 μM and 6 μM. The detection efficiency of the method was confirmed by ion exchange chromatography. Moreover, the specificity of the sensor was evaluated and no cross-reactivity from nontarget analytes was observed. This method was successfully applied for the direct analysis of histamine in white wine providing detection limit much lower than the histamine maximum levels established by EU regulation in food samples. The recovery rate was excellent, ranging from 84 to 100% with an RSD of less than 4.0%. The main advantage of the proposed method is full automation of the analytical procedure that reduces the time and cost of the analysis, solvent consumption and sample manipulation, enabling routine analysis of large numbers of samples for histamine and highly accurate and precise results.
Author Milica Gagic - Mendel University in Brno
Milica Gagic,,
, Lukas Nejdl - Mendel University in Brno
Lukas Nejdl,,
, Kledi Xhaxhiu - Agricultural University of Tirana
Kledi Xhaxhiu,,
, Natalia Cernei - Mendel University in Brno
Natalia Cernei,,
, Ondrej Zitka - Mendel University in Brno
Ondrej Zitka,,
, Ewelina Jamróz (FoFT / IoCh)
Ewelina Jamróz,,
- Institute of chemistry
, Pavel Svec - Mendel University in Brno
Pavel Svec,,
, Lukas Richtera - Mendel University in Brno
Lukas Richtera,,
, Pavel Kopel - Palacky University (UP v Olomouci)
Pavel Kopel,,
, Vedran Milosavljević - Mendel University in Brno
Vedran Milosavljević,,
et al.`
Journal seriesTalanta, ISSN 0039-9140, e-ISSN 1873-3573, (N/A 100 pkt)
Issue year2020
Publication size in sheets0.5
Article number120789
Keywords in EnglishHistamine, Maghemite, Quantum dots, Food safety, Ion exchange chromatography
ASJC Classification1600 General Chemistry
URL https://www.sciencedirect.com/science/article/pii/S0039914020300801/pdfft?md5=236793b1f7b1cdc8bf22fae712187edd&pid=1-s2.0-S0039914020300801-main.pdf
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.27; WoS Impact Factor: 2018 = 4.916 (2) - 2018=4.322 (5)
Citation count*
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FinansowanieThis work was supported by the ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314), and CEITEC 2020 (LQ1601) from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II which is highly acknowledged.
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