Leucojum aestivum plants propagated in in vitro bioreactor culture and on solid media containing cytokinins
Agata Ptak , Magdalena Simlat , Michał Kwiecień , Dominique Laurain-Mattar
AbstractCytokinins are growth regulators that stimulate cell division and control morphogenesis in plants, however their role in regulating secondary metabolism is not well studied. The influence of various cytokinins (benzyladenine, zeatin, kinetin, meta-topolin, thidiazuron) and culture systems (solid and temporary immersion RITA® system) on the quality Leucojum aestivum plant regenerated from somatic embryos was investigated. The largest number of regenerated plants (181.6 and 168.8) was obtained from the embryos cultivated on media enriched with meta-topolin and benzyladenine. Thidiazuron and meta-topolin led to the highest number of normally developed plants (94.8 and 90.6). The random amplified polymorphic DNA analysis of in vitro and in vivo plants showed four clusters of similarity. The highest biomass (growth index: 2.49) was obtained with the temporary immersion RITA® system. Alkaloid extracts were analyzed by LC-MS, leading to the quantification of galanthamine and lycorine both in plant materials and in liquid media. The highest contents of galanthamine (0.05% dry weight) were observed in plants cultivated in the presence of thidiazuron in bioreactor system. Galanthamine was accumulated (highest content 0.05% dry weight) in plants cultivated in the presence of thidiazuron in bioreactor system whereas lycorine was synthetized mainly in plants cultivated on solid media.
|Journal series||Engineering in Life Sciences, ISSN 1618-0240, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Cytokinins, Galanthamine, Lycorine, Random amplified polymorphic DNA (RAPD), Temporary immersion system RITA®|
|ASJC Classification||; ;|
|Publication indicators||= 14; : 2013 = 0.895; : 2013 = 1.89 (2) - 2013=2.049 (5)|
|Citation count*||6 (2015-09-27)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.