Leucojum aestivum plants propagated in in vitro bioreactor culture and on solid media containing cytokinins

Agata Ptak , Magdalena Simlat , Michał Kwiecień , Dominique Laurain-Mattar

Abstract

Cytokinins are growth regulators that stimulate cell division and control morphogenesis in plants, however their role in regulating secondary metabolism is not well studied. The influence of various cytokinins (benzyladenine, zeatin, kinetin, meta-topolin, thidiazuron) and culture systems (solid and temporary immersion RITA® system) on the quality Leucojum aestivum plant regenerated from somatic embryos was investigated. The largest number of regenerated plants (181.6 and 168.8) was obtained from the embryos cultivated on media enriched with meta-topolin and benzyladenine. Thidiazuron and meta-topolin led to the highest number of normally developed plants (94.8 and 90.6). The random amplified polymorphic DNA analysis of in vitro and in vivo plants showed four clusters of similarity. The highest biomass (growth index: 2.49) was obtained with the temporary immersion RITA® system. Alkaloid extracts were analyzed by LC-MS, leading to the quantification of galanthamine and lycorine both in plant materials and in liquid media. The highest contents of galanthamine (0.05% dry weight) were observed in plants cultivated in the presence of thidiazuron in bioreactor system. Galanthamine was accumulated (highest content 0.05% dry weight) in plants cultivated in the presence of thidiazuron in bioreactor system whereas lycorine was synthetized mainly in plants cultivated on solid media.
Author Agata Ptak (FoAE / DoPBaS)
Agata Ptak,,
- Department of Plant Breeding and Seed
, Magdalena Simlat (FoAE / DoPBaS)
Magdalena Simlat,,
- Department of Plant Breeding and Seed
, Michał Kwiecień (FoAE / DoPBaS)
Michał Kwiecień,,
- Department of Plant Breeding and Seed
, Dominique Laurain-Mattar
Dominique Laurain-Mattar,,
-
Journal seriesEngineering in Life Sciences, ISSN 1618-0240, (A 25 pkt)
Issue year2013
Vol13
No3
Pages261-270
Publication size in sheets0.5
Keywords in EnglishCytokinins, Galanthamine, Lycorine, Random amplified polymorphic DNA (RAPD), Temporary immersion system RITA®
ASJC Classification1502 Bioengineering; 2305 Environmental Engineering; 1305 Biotechnology
DOIDOI:10.1002/elsc.201200109
URL http://onlinelibrary.wiley.com/doi/10.1002/elsc.201200109/full
Internal identifierWRE/2013/20
Languageen angielski
Score (nominal)25
Score sourcejournalList
Publication indicators WoS Citations = 14; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.895; WoS Impact Factor: 2013 = 1.89 (2) - 2013=2.049 (5)
Citation count*6 (2015-09-27)
Additional fields
FinansowanieDS/3129/KHRiN
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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