MicroRNAs in Vitis vinifera cv. Chardonnay are differentially expressed in response to Diaporthe Species

Aleš Eichmeier , Tomas Kiss , Eliška Peňázová , Jakub Pečenka , Akila Berraf-Tebbal , Miroslav Baranek , Robert Pokluda , Jana Cechova , David Gramaje , Dariusz Grzebelus

Abstract

Diaporthe species are important pathogens, saprobes, and endophytes on grapevines. Several species are known, either as agents of pre- or post-harvest infections, as causal agents of many relevant diseases, including swelling arm, trunk cankers, leaf spots, root and fruit rots, wilts, and cane bleaching. A growing body of evidence exists that a class of small non-coding endogenous RNAs, known as microRNAs (miRNAs), play an important role in post-transcriptional gene regulation, during plant development and responses to biotic and abiotic stresses. In this study, we explored differentially expressed miRNAs in response to Diaporthe eres and Diaporthe bohemiae infection in Vitis vinifera cv. Chardonnay under in vitro conditions. We used computational methods to predict putative miRNA targets in order to explore the involvement of possible pathogen response pathways. We identified 136 known and 41 new miRNA sequence variants, likely generated through post-transcriptional modifications. In the Diaporthe eres treatment, 61 known and 17 new miRNAs were identified while in the Diaporthe bohemiae treatment, 101 known and 21 new miRNAs were revealed. Our results contribute to further understanding the role miRNAs play during plant pathogenesis, which is possibly crucial in understanding disease symptom development in grapevines infected by D. eres and D. bohemiae.
Author Aleš Eichmeier
Aleš Eichmeier,,
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, Tomas Kiss
Tomas Kiss,,
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, Eliška Peňázová
Eliška Peňázová,,
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, Jakub Pečenka
Jakub Pečenka,,
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, Akila Berraf-Tebbal
Akila Berraf-Tebbal,,
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, Miroslav Baranek
Miroslav Baranek,,
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, Robert Pokluda
Robert Pokluda,,
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, Jana Cechova
Jana Cechova,,
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, David Gramaje
David Gramaje,,
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, Dariusz Grzebelus (FoBaH / IoPBaB)
Dariusz Grzebelus,,
- Institute of Plant Biology and Biotechnology
Journal seriesGenes, ISSN 2073-4425, (N/A 100 pkt)
Issue year2019
Vol10
No11
Pages1-20
Publication size in sheets0.95
Article number905
Keywords in EnglishHigh-throughput sequencing; grapevine; RT-qPCR; miRNA
ASJC Classification2716 Genetics(clinical); 1311 Genetics
DOIDOI:10.3390/genes10110905
URL https://www.mdpi.com/2073-4425/10/11/905/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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MicroRNAs in Vitis vinifera cv. Chardonnay Are Differentially Expressed in Response to Diaporthe Species of 13-11-2019
1,43 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.864; WoS Impact Factor: 2018 = 3.331 (2) - 2018=3.484 (5)
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FinansowanieThis work was supported from ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314). This research was also supported by the project No. TJ02000096
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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