Analysis of sperm chromatin structure in blue foxes (Alopex lagopus) and silver foxes (Vulpes vulpes)

Marta Kuchta-Gładysz , Katarzyna Andraszek , Olga Szeleszczuk , Piotr Niedbała , Agnieszka Otwinowska-Mindur

Abstract

The latest reports indicate that sperm function is significantly influenced by the chromatin structure of the sperm and the integrity of its DNA. The aim of the study was to determine the level of condensation in the chromatin structure of fresh semen and the degree of damage to the DNA chain in fresh and chilled semen of farmed blue and silver foxes. The sperm of 15 blue foxes and 18 silver foxes at the age of one year was used in the study. The chromatin structure was evaluated by three staining techniques: aniline blue, chromomycin A3 and acridine orange. A comet assay was used to assess sperm DNA integrity in fresh and chilled semen. The average percentage of head DNA changed over time: in blue foxes from 99.12% to 97.83%, and in silver foxes from 99.37% to 98.05%. Highly significant differences (P < 0.01) in head DNA were found between species. Within each species, highly significant differences (P < P < 0.01) were found in the chilled semen (0 h/24 h/48 h/72 h). After staining with aniline blue (AB), chromomycin A3 (CMA3) and acridine orange (AO), most sperm had normal histone retention and a stable, native DNA structure. In blue foxes, the average percentage of sperm with elevated, abnormal histone content, sperm with impaired protamination, and sperm with DNA fragmentation was 1–13%; 1–16% and 0%, respectively. The percentages for silver foxes were similar (3–17%, 5–17% and 2%).
Author Marta Kuchta-Gładysz (FoAS / DoARaA)
Marta Kuchta-Gładysz,,
- Department of Animal Reproduction and Anatomy
, Katarzyna Andraszek - Siedlce University of Natural Sciences and Humanities
Katarzyna Andraszek,,
-
, Olga Szeleszczuk (FoAS / DoARaA)
Olga Szeleszczuk,,
- Department of Animal Reproduction and Anatomy
, Piotr Niedbała (FoAS / DoARaA)
Piotr Niedbała,,
- Department of Animal Reproduction and Anatomy
, Agnieszka Otwinowska-Mindur (DoAS / DoGaAIM)
Agnieszka Otwinowska-Mindur,,
- Department of Genetics and Animal Improvement Methods
Journal seriesLivestock Science, ISSN 1871-1413, e-ISSN 1878-0490, (N/A 140 pkt)
Issue year2020
Vol231
No103869
Pages1-8
Publication size in sheets0.5
Keywords in EnglishFoxes, Sperm, Comet assay, Protamine, Histone
ASJC Classification3400 General Veterinary; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.livsci.2019.103869
URL https://www.sciencedirect.com/science/article/pii/S1871141319306596/pdfft?md5=e73cdd01fbe0537e7fe297aa9c56f7f6&pid=1-s2.0-S1871141319306596-main.pdf
Languageen angielski
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Analysis of sperm chromatin structure in blue foxes (Alopex lagopus) and silver foxes (Vulpes vulpes) of 03-06-2020
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Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.036; WoS Impact Factor: 2018 = 1.376 (2) - 2018=1.747 (5)
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