Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

Sabina Malaga , Anna Janeczko , Franciszek Jankowiak , Piotr Waligórski , Jana Oklestkova , Ewa Dubas , Monika Krzewska , Anna Nowicka , Ewa Surówka , Marcin Rapacz , Magdalena Wójcik-Jagła , Przemysław Kopeć , Tomasz Hura , Agnieszka Ostrowska , Katarzyna Kaczanowska , Iwona Żur

Abstract

Ten doubled haploid (DH) lines of winter barley with an increased range of freezing/drought tolerance were used to identify phytohormones involved in plant stress acclimation. Cold hardening and drought stress were applied at the most critical stages of plant development on young seedlings and heading plants, respectively. The level of the phytohormones was significantly higher at heading, more than 5-fold in respect of salicylic acid (SA) and total brassinosteroids (BRs) and 1.7-fold in the case of abscisic acid (ABA). Moreover, the spectrum of detectable BRs increased from one—homocastasterone (HCS)—found in seedlings to four BRs identified in heading plants [HCS, castasterone (CS), teasterone and dolicholide], with the last one detected for the first time in cereal species. To some extent freezing tolerance seems to be determined by native hormonal status as control seedlings of tolerant DH lines contained 1.4- and 2.3-fold lower amount of ABA and HCS and 2.3-fold higher amount of SA in comparison to freezing-sensitive ones. Such dependency was not observed in heading plants as significant variation in CS content was the only detected difference. Under stress treatments, tolerant DH lines accumulated significantly lower (75–81%) amount of ABA, which probably reflected lower stress intensity resulting from another defence strategy. In contrast, stress-induced significant almost 2-fold increase in HCS/CS and 2–3-fold decrease in SA content specific for tolerant DH lines of barley suggest the involvement of these molecules in freezing/drought defence. Detected correlations suggest their interaction with nonspecific peroxidase and low molecular weight antioxidants.
Author Sabina Malaga
Sabina Malaga,,
-
, Anna Janeczko
Anna Janeczko,,
-
, Franciszek Jankowiak
Franciszek Jankowiak,,
-
, Piotr Waligórski
Piotr Waligórski,,
-
, Jana Oklestkova
Jana Oklestkova,,
-
, Ewa Dubas
Ewa Dubas,,
-
, Monika Krzewska
Monika Krzewska,,
-
, Anna Nowicka
Anna Nowicka,,
-
, Ewa Surówka
Ewa Surówka,,
-
, Marcin Rapacz (FoAaE / DoPP)
Marcin Rapacz,,
- Department of Plant Physiology
et al.`
Journal seriesPlant Growth Regulation, ISSN 0167-6903, e-ISSN 1573-5087, (N/A 70 pkt)
Issue year2020
Vol90
No1
Pages173-188
Publication size in sheets0.75
Keywords in EnglishEnvironmental stress acclimation, Homozygous lines, Hordeum vulgare (L.), Phytohormones
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science; 1314 Physiology
DOIDOI:10.1007/s10725-019-00544-9
URL https://link.springer.com/content/pdf/10.1007/s10725-019-00544-9.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley of 08-01-2020
1,65 MB
Score (nominal)70
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.001; WoS Impact Factor: 2018 = 2.473 (2) - 2018=2.758 (5)
Citation count*
Additional fields
FinansowanieThe research was performed in the frame of the Project HOR.hn.802.15.2018, No. 26 financed by the Polish Ministry of Agriculture and Rural Development within the Program ‘Basic Research for Biological Progress in Plant Production’. The analysis of BRs was supported from the European Regional Development Funding Project ‘Plants as a Tool for Sustainable Global Development’ (No. CZ.02.1.01/0.0/0.0/16_019/0000827).
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?