Genetic variability of Chalara fraxinea, dieback cause of European ash (Fraxinus excelsior L.)

Wojciech Kraj , Marcin Zarek , Tadeusz Kowalski

Abstract

A total of 159 colonies of Chalara fraxinea were isolated between 2005 and 2006 from dying trees of European ash (Fraxinus excelsior L.) aged between 3 and 10 years. They derived from five regions of Poland differing by geographic location and climatic conditions. On the basis of 90 RAMS markers, pathogen intra- and inter-population variability, as well as its dependency on geographic distance and climatic conditions in the regions of strain origin, was analysed. The applied measures of intrapopulation genetic variability (genetic distance, Nei’s unbiased diversity, Shannon’s Information Index and percentage of polymorphic loci) allowed for differentiation of two strain groups: the first deriving from lowlands and the second from uplands and mountainous areas. Strains in lowlands were characterised by smaller number of markers, smaller number of polymorphic loci and smaller intrapopulation genetic variability. Positive and statistically significant correlation was shown between variability of isolates and elevation of regions above sea level. Pair-wise genetic distances between groups of isolates (Nei’s unbiased genetic distance) from particular regions were not significantly correlated with the corresponding geographic distances. On the basis of AMOVA, it was shown that 85% of variability was within-region differences and 2% between-region differences, whereas differences between lowlands and uplands were 13%. Principal Components Analysis (PCA) for the investigated regions confirmed the results from Nei’s genetic distance matrix.
Author Wojciech Kraj (FoF / FEPI / DoFFMaPoT)
Wojciech Kraj,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
, Marcin Zarek (FoF / FEPI / DoFFMaPoT)
Marcin Zarek,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
, Tadeusz Kowalski (FoF / FEPI / DoFFMaPoT)
Tadeusz Kowalski,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
Journal seriesMycological Progress, ISSN 1617-416X, e-ISSN 1861-8952, (A 20 pkt)
Issue year2012
Vol11
No1
Pages37-45
Publication size in sheets0.5
Keywords in EnglishChalara fraxinea, Genetic variability, Tree diseases, RAMS markers, Population genetics
ASJC Classification1110 Plant Science
DOIDOI:10.1007/s11557-010-0724-z
URL https://link.springer.com/article/10.1007/s11557-010-0724-z
Languageen angielski
Score (nominal)20
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2012 = 1.024; WoS Impact Factor: 2012 = 1.606 (2) - 2012=1.62 (5); WoS Citations = 30
Citation count*32 (2016-05-15)
Additional fields
FinansowanieThis study was financially supported by The Ministry of Science and Higher Education, grant No. N N309 146937
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?