Plasma membrane ATPase and the aquaporin HvPIP1 in barley brassinosteroid mutants acclimated to high and low temperature

Iwona Sadura , Marta Libik-Konieczny , Barbara Jurczyk , Damian Gruszka , Anna Janeczko

Abstract

The integral parts of the cell membranes are the functional proteins, which are crucial for cell life. Among them, proton-pumping ATPase and aquaporins appear to be of particular importance. There is some knowledge about the effect of the temperature during plant growth, including stress-inducing temperatures, on the accumulation of the membrane proteins: plasma membrane H+-ATPase and aquaporins, but not much is known about the effect of the phytohormones (i.e. brassinosteroids (BR)) on control of accumulation of these proteins. The aim of our study was to answer the question of how a BR deficit and disturbances in the BR perception/signalling affect the accumulation of plasma membrane H+-ATPase (PM H+-ATPase), the aquaporin HvPIP1 transcript and protein in barley growing at 20 °C and during its acclimation at 5 °C and 27 °C. For the studies, the BR-deficient mutant 522DK (derived from the wild-type Delisa), the BR-deficient mutant BW084 and the BR-signalling mutant BW312 and their wild-type Bowman were used. Generally, temperature of growth was significant factor influencing on the level of the accumulation of the H+-ATPase and HvPIP1 transcript and the PM H+-ATPase and HvPIP1 protein in barley leaves. The level of the accumulation of the HvPIP1 transcript decreased at 5 °C (compared to 20 °C), but was higher at 27 °C than at 20 °C in the analyzed cultivars. In both cultivars the protein HvPIP1 was accumulated in the highest amounts at 27 °C. On the other hand, the barley mutants with a BR deficiency or with BR signalling disturbances were characterised by an altered accumulation level of PM H+- ATPase, the aquaporin HvPIP1 transcript and protein (compared to the wild types), which may suggest the involvement of brassinosteroids in regulating PM H+-ATPase and aquaporin HvPIP1 at the transcriptional and translational levels.
Author Iwona Sadura - [Polska Akademia Nauk]
Iwona Sadura,,
-
- Polska Akademia Nauk
, Marta Libik-Konieczny - [Polska Akademia Nauk]
Marta Libik-Konieczny,,
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- Polska Akademia Nauk
, Barbara Jurczyk (FoAaE / DoPoPBaSP)
Barbara Jurczyk,,
- Department of Physiology of Plant Breeding and Seed Production
, Damian Gruszka - [Uniwersytet Śląski ]
Damian Gruszka,,
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- Uniwersytet Śląski
, Anna Janeczko - [Polska Akademia Nauk]
Anna Janeczko,,
-
- Polska Akademia Nauk
Journal seriesJournal of Plant Physiology, ISSN 0176-1617, e-ISSN 1618-1328, (N/A 100 pkt)
Issue year2020
Vol244
Pages1-8
Publication size in sheets0.5
Keywords in EnglishAcclimation, Brassinosteroids, PM H+-ATPase, High temperatures, HvPIP1, Low temperatures
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science; 1314 Physiology
DOIDOI:10.1016/j.jplph.2019.153090
URL https://www.sciencedirect.com/science/article/pii/S0176161719302196?via%3Dihub
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Plasma membrane ATPase and the aquaporin HvPIP1 in barley brassinosteroid mutants acclimated to high and low temperature of 21-05-2020
1,59 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.174; WoS Impact Factor: 2018 = 2.825 (2) - 2018=3.236 (5)
Citation count*
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FinansowanieThe work was funded by a grant from the National Science Centre (2016–2018) No. 2015/17/B/NZ9/01695 (Poland).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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