Genetic Engineering of Carrot
Rafał Barański , Aneta Łukasiewicz
AbstractCarrot (Daucus carota) is one of the model species used in research for in vitro plant cell and tissue culture. The development of these techniques has enabled efficient cell and tissue proliferation and somatic embryogenesis under in vitro conditions, thus favoring the use of carrot for elucidating the mechanisms of horizontal gene transfer and gene function. Deployment of genetic engineering techniques has led to the development of carrots with improved traits, enhancing plant production for human health. The first product derived from genetically modified (GM) carrot cells cultured in a bioreactor has been approved for the treatment of human metabolic disease and for commercialization. This chapter describes methods of carrot genetic transformation using both vector and non-vector methods. Furthermore, we present reports of basic research in which carrot was used as a model to elucidate the function of heterologous genes and promoters, revealing selected mechanisms of plant metabolism, including the phenomenon of bacteria to plant gene transfer. Separate sections exemplify modified characteristics of GM carrot, including resistance to pathogens and the biosynthesis of recombinant proteins.
|Publication size in sheets||1.85|
|Book||Simon Philipp, Iorizzo Massimo , Grzebelus Dariusz, Barański Rafał (eds.): The Carrot Genome, Compendium of Plant Genomes, 2019, Springer, ISBN 978-3-030-03388-0, [978-3-030-03389-7], 372 p., DOI:10.1007/978-3-030-03389-7|
|Keywords in English||Agrobacterium tumefaciens, Rhizobium rhizogenes, Daucus carota, Genetic transformation, Non-vector methods, Transgenic plants, Genetically modified organism|
|Publication indicators||= 0|
|Citation count*||2 (2020-04-09)|
|Finansowanie||The support of the Ministry of Science and Higher Education of the Republic of Poland is gratefully acknowledged.|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.