The effect of triclosan on hormone secretion and viability of human choriocarcinoma JEG-3 cells

Ewelina Honkisz , Dorota Zięba-Przybylska , Anna Wójtowicz

Abstract

Triclosan is an antimicrobial agent frequently used in pharmaceuticals and personal care products. We analyzed triclosan for its action on placental secretion of progesterone, estradiol and human chorionic gonadotropin in vitro in the JEG-3 cells. We also investigated its action on cell viability, proliferation and apoptosis. The JEG-3 cells were cultured with increasing doses of triclosan (1×10(-9)-1×10(-4) M) for 24, 48 and 72 h. Triclosan was found to increase estradiol and progesterone secretion after short- and long-term exposure. The stimulatory effect was observed up to 10 μM after short- and long-term exposure to triclosan. In addition, triclosan caused an adverse effect on β-hCG secretion. The highest doses of triclosan (50 and 100 μM) showed a strong cytotoxic effect. Anti proliferative and pro-apoptotic effects were also observed. Overall, this study demonstrates that triclosan may indirectly disrupt steroidogenesis which may, in turn, affect placental development and consequently fetal growth.
Author Ewelina Honkisz (DoAS / DoAB)
Ewelina Honkisz,,
- Department of Animal Biotechnology
, Dorota Zięba-Przybylska (DoAS / HBZ-KHTChMP)
Dorota Zięba-Przybylska,,
- Department of Swine and Small Ruminant Breeding
, Anna Wójtowicz (DoAS / HBZ-KHTChMP)
Anna Wójtowicz,,
- Department of Swine and Small Ruminant Breeding
Journal seriesReproductive Toxicology, ISSN 0890-6238, (A 35 pkt)
Issue year2012
DOIDOI:10.1016/j.reprotox.2012.05.094
URL http://www.ncbi.nlm.nih.gov/pubmed/22677473
Languageen angielski
Score (nominal)35
Publication indicators WoS Citations = 42
Citation count*26 (2016-06-20)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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