Selected Aspects of Iodate and Iodosalicylate Metabolism in Lettuce Including the Activity of Vanadium Dependent Haloperoxidases as Affected by Exogenous Vanadium

Sylwester Smoleń , Iwona Kowalska , Mariya Halka , Iwona Ledwożyw-Smoleń , Marlena Grzanka , Łukasz Skoczylas , Małgorzata Czernicka , Joanna Pitala

Abstract

In marine algae, vanadium (V) regulates the cellular uptake of iodine (I) and its volatilization as I2, the processes catalyzed by vanadium-dependent haloperoxidases (vHPO). Relationships between I and vanadium V in higher plants, including crop plants, have not yet been described. Little is known about the possibility of the synthesis of plant-derived thyroid hormone analogs (PDTHA) in crop plants. The activity of vHPO in crop plants as well as the uptake and metabolism of iodosalicylates in lettuce have not yet been studied. This studyaimed to determine the effect of V on the uptake and accumulation of various forms of I, the metabolism of iodosalicylates and iodobenzoates and, finally, on the accumulation of T3 (triiodothyronine—as example of PDTHA) in plants. Lettuce (Lactuca sativa L. var. capitata ‘Melodion’ cv.) cultivation in a hydroponic NutrientFilm Technique (NFT) system was conducted with the introduction of 0 (control), 0.05, 0.1, 0.2, and 0.4 µM V doses of ammonium metavanadate (NH4VO3) in four independent experiments. No iodine treatment was applied in Experiment No. 1, while iodine compounds were applied at a dose of 10 µM (based on our own previous research) as KIO3, 5-iodosalicylic acid (5-ISA) and 3,5-diiodosalicylic acid (3,5-diISA) in Experiment Nos. 2, 3 and 4, respectively. When lettuce was grown at trace amount of I in the nutrient solution, increasing doses of V contributed to the increase of (a) I content in roots, (b) I uptake by whole lettuce plants (leaves + roots), and (c) vHPO activity in leaves (for doses 0.05–0.20 µM V). Vanadium was mainly found in roots where the content of this element increased proportionally to its dose. The content of V in leaves was not modified by V introduced into the nutrient solution. We found that5-ISA, 3,5-diISA and T3 were naturally synthesized in lettuce and its content increased when 5-ISA, 3,5-diISA were applied. Quantitative changes in the accumulation of organic metabolites (iodosalicylates and iodobenzoates) accumulation were observed, along with increased T3 synthesis, with its content in leaves exceeding the level of individual iodosalicylates and iodobenzoates. The content of T3 was not affected by V fertilization. It was concluded that iodosalicylates may participate in the biosynthesis pathway of T3—and probably of other PDTHA compounds.
Author Sylwester Smoleń (FoBaH / IoPBaB)
Sylwester Smoleń,,
- Institute of Plant Biology and Biotechnology
, Iwona Kowalska (FoBaH / IoPBaB)
Iwona Kowalska,,
- Institute of Plant Biology and Biotechnology
, Mariya Halka (FoBaH / IoPBaB)
Mariya Halka,,
- Institute of Plant Biology and Biotechnology
, Iwona Ledwożyw-Smoleń (FoBaH / IoPBaB / DoB)
Iwona Ledwożyw-Smoleń,,
- Department of Biochemistry
, Marlena Grzanka (FoBaH / IoPBaB)
Marlena Grzanka,,
- Institute of Plant Biology and Biotechnology
, Łukasz Skoczylas (FoFT / DoToFVaM)
Łukasz Skoczylas,,
- Department of Technology of Fruits, Vegetables and Mushrooms
, Małgorzata Czernicka (FoBaH / IoPBaB / DoGPBaSP)
Małgorzata Czernicka,,
- Department of Genetics, Plant Breeding and Seed Production
, Joanna Pitala (FoBaH / IoPBaB)
Joanna Pitala,,
- Institute of Plant Biology and Biotechnology
Journal seriesAgronomy, [Agronomy], ISSN 2073-4395, e-ISSN 2073-4395, (N/A 100 pkt)
Issue year2020
Vol10
No1
Pages1-21
Publication size in sheets1
Keywords in Englishbeneficial elements; iodobenzoates; iodosalicylates; plant-derived thyroid hormone analogs; T3; thyroid hormone; triiodothyronine; vanadium-dependent haloperoxidases
ASJC Classification1102 Agronomy and Crop Science
DOIDOI:10.3390/agronomy10010001
URL https://www.mdpi.com/2073-4395/10/1/1
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Selected Aspects of Iodate and Iodosalicylate Metabolism in Lettuce Including the Activity of Vanadium Dependent Haloperoxidases as Affected by Exogenous Vanadium of 18-12-2019
3,06 MB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.272; WoS Impact Factor: 2018 = 2.259 (2)
Citation count*
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FinansowanieThis research was financed by the National Science Centre, Poland (Grant No. UMO-2017/25/B/NZ9/00312).
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