Aquaporin 4 in the chicken oviduct during a pause in laying induced by food deprivation

Anna Hrabia , Joanna Socha , Noboru Saito , Małgorzata Grzesiak , Andrzej Sechman

Abstract

In the present study we hypothesize that aquaporin 4 (AQP4) expression in the chicken oviduct would change during a pause in egg laying that was induced by fasting. Accordingly, the aim of this investigation was to examine the AQP4 mRNA and protein expression, and immunolocalization in the chicken oviduct during the course of regression. The experiment was carried out on laying hens subjected to a pause in laying that was induced by food deprivation for 5 days. Control hens were fed ad libitum. The birds were sacrificed on day 6 of the experiment and all segments of the oviduct were isolated, including the infundibulum, magnum, isthmus, shell gland, and vagina. Subsequently, the gene and protein expressions of AQP4 in the tissues were tested by real-time PCR and Western blot, respectively. The relative mRNA expression of AQP4 was the highest in the infundibulum and vagina and the lowest, and least detectable, in the magnum. The level of AQP4 protein was the highest in the infundibulum and the lowest in the magnum. Fasting resulted in a decrease of the AQP4 mRNA expression () in the infundibulum, a decrease in protein abundance () in the shell gland, and an increase in protein level () in the vagina. Immunohistochemistry demonstrated tissue- and cell-dependent localization of AQP4 protein in the oviductal wall. The intensity of staining was as follows: the infundibulum shell gland vagina isthmus ≫ magnum. In the control hens, the immunoreactivity for AQP4 in the vagina was similar, whereas in other oviductal segments, the immunoreactivity was stronger when compared with the chickens subjected to a pause in laying. In summary, these findings suggest that the AQP4 is an essential protein involved in the regulation of water transport required to create a proper microenvironment for fertilization and egg formation in the hen oviduct.
Author Anna Hrabia (FoAS / Department of Animal Physiology and Endocrinology)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Joanna Socha (FoAS / Department of Animal Physiology and Endocrinology)
Joanna Socha,,
- Department of Animal Physiology and Endocrinology
, Noboru Saito - Okayama University
Noboru Saito,,
-
, Małgorzata Grzesiak (FoAS / DoAPaE) - Jagiellonian University (UJ)
Małgorzata Grzesiak,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (FoAS / Department of Animal Physiology and Endocrinology)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Other language title versionsAquaporine 4 dans l’oviducte de poule lors d’une pause de ponte induite par une privation alimentaire
Journal seriesComptes Rendus Biologies, [Comptes Rendus - Biologies], ISSN 1631-0691, e-ISSN 1768-3238, (N/A 70 pkt)
Issue year2020
Vol343
No1
Pages89-99
Publication size in sheets0.5
Keywords in EnglishAQP4, Pause in laying, Fasting, Oviduct, Chicken
ASJC Classification2700 General Medicine; 1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology; 2400 General Immunology and Microbiology
DOIDOI:10.5802/crbiol.5
URL https://comptes-rendus.academie-sciences.fr/biologies/article/CRBIOL_2020__343_1_89_0.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Aquaporin 4 in the chicken oviduct during a pause in laying induced by food deprivation of 16-06-2020
1,13 MB
Score (nominal)70
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.73; WoS Impact Factor: 2018 = 1.867 (2) - 2018=1.556 (5)
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FinansowanieThis work was supported by the Ministry of Sciences and Higher Education, Poland (grant number 0215000000-D204).
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