Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation

Ewa Pociecha , Franciszek Janowiak , Ewa Dubas , Iwona Żur , Krzysztof Tokarz , Irena Kolasińska , Agnieszka Płażek

Abstract

Resistance to snow mould is a feature determined by multiple genes. Therefore, determining the phenotype of resistant plants is difficult as it requires an investigation over a long period of time from cold acclimation through pathogenesis. The aim of the present study was (i) to determine the characteristics of the resistant genotype and (ii) to clarify the connections between photosynthesis during cold acclimation and then pathogenesis caused by Microdochium nivale. Two inbred lines of winter rye (Secale cereale L.) differing in their susceptibility to snow mould were used in the study. After cold acclimation snow mould resistant (SMR) line was characterised by higher values of CO2 assimilation and electron transport efficiency but did not differ from snow mould susceptible (SMS) line in carboxylation rate of RuBisCO (Vcmax). Higher soluble carbohydrate accumulation, due to higher photosynthesis intensity, as well as an ABA increase at 5 days post infection (DPI) in leaves and crowns were found in SMR line during the pathogenesis period. Callose deposition was found around non-infected bundle sheets and in cortex cells at 5 DPI (at the same time point as ABA peak) only in SMR line, which probably prevented the infection of leaf initials. Early leaf initials infection in SMS line may be responsible for inhibiting leaf growth and plant regeneration after stress cessation. The results show different physiological and biochemical characteristics of the investigated lines, which can be applied in the selection of resistant genotypes and identifying genomic regions responsible for metabolic pathways increasing pathogen resistance.
Author Ewa Pociecha (FoAaE / DoPP)
Ewa Pociecha,,
- Department of Plant Physiology
, Franciszek Janowiak
Franciszek Janowiak,,
-
, Ewa Dubas
Ewa Dubas,,
-
, Iwona Żur
Iwona Żur,,
-
, Krzysztof Tokarz (FoH / DoBaPP)
Krzysztof Tokarz,,
- Department of Botany and Plant Physiology
, Irena Kolasińska
Irena Kolasińska,,
-
, Agnieszka Płażek (FoAaE / DoPP)
Agnieszka Płażek,,
- Department of Plant Physiology
Journal seriesPlant Physiology and Biochemistry, ISSN 0981-9428, (A 35 pkt)
Issue year2013
Vol70
Pages360-367
Publication size in sheets0.5
Keywords in Englishabscisic acid, cold acclimation, microdochium nivale, photosynthesis, phytopathogenesis, total soluble carbohydrates
ASJC Classification1110 Plant Science; 1311 Genetics; 1314 Physiology
DOIDOI:10.1016/j.plaphy.2013.05.033
URL http://www.sciencedirect.com/science/article/pii/S0981942813002088
Internal identifierWRE/2013/150
Languageen angielski
Score (nominal)35
Score sourcejournalList
Publication indicators WoS Citations = 7; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.369; WoS Impact Factor: 2013 = 2.352 (2) - 2013=3.051 (5)
Citation count*5 (2016-08-08)
Additional fields
FinansowanieABA Projekt 592/N-COST/2009/0; MNiSW Action COST FA0604
OświadczenieK. Tokarz.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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