The meaning of non-classical estrogen receptors and peroxisome proliferator-activated receptor for boar Leydig cell of immature testis

Małgorzata Kotula-Balak , Michal Duliban , Piotr Pawlicki , Ryszard Tuz , Barbara Bilińska , Bartosz Płachno , Zbigniew Arent , Izabela Krakowska , Kazimierz Tarasiuk


Communication in biological systems involves diverse-types of cell-cell interaction including cross-talk between receptors expressed by the target cells. Recently, novel sort of estrogen receptors (G protein - coupled estrogen receptor; GPER and estrogen-related receptor; ERR) that signal directly via estrogen binding and/or via mutual interaction-regulated estrogen signaling were reported in various organs including testis. Peroxisome proliferator - activated receptor (PPAR) is responsible for maintaining of lipid homeostasis that is critical for sex steroid production in the testis. Here, we investigated the role of interaction between GPER, ERRβ and PPARγ in steroidogenic Leydig cells of immature boar testis. Testicular fragments cultured ex vivo were treated with GPER or PPARγ antagonists. Then, cell ultrastructure, expression and localization of GPER, ERRβ, PPARγ together with the molecular receptor mechanism, through cyclic AMP and Raf/Ras/extracellular signal activated kinases (ERK), in the control of cholesterol concentration and estrogen production by Leydig cells were studied. In the ultrastructure of antagonist-treated Leydig cells, mitochondria were not branched and not bifurcated as they were found in control. Additionally, in PPARγ-blocked Leydig cells changes in the number of lipid droplets were revealed. Independent of used antagonist, western blot revealed decreased co-expression of GPER, ERRβ, PPARγ with exception of increased expression of ERRβ after PPARγ blockage. Immunohistochemistry confirmed presence of all receptors partially located in the nucleus or cytoplasm of Leydig cells of both control and treated testes. Changes in receptor expression, decreased cholesterol and increased estradiol tissue concentrations occurred through decreased cAMP level (with exception after GPER blockage) as well as Raf/Ras/ERK pathway expression. These all findings indicate that GPER-ERRβ-PPARγ interaction exists in immature boar testis and regulates Leydig cell function. Further detailed studies and considerations on GPER-ERRβ-PPARγ as possible diagnosis/therapy target in disturbances of testis steroidogenic function are needed.
Author Małgorzata Kotula-Balak (VET)
Małgorzata Kotula-Balak,,
- University Centre for Veterinary Medicine UJ-UR
, Michal Duliban - Jagiellonian University (UJ)
Michal Duliban,,
, Piotr Pawlicki - Jagiellonian University (UJ)
Piotr Pawlicki,,
, Ryszard Tuz (FoAS / IoAS)
Ryszard Tuz,,
- Institute of Animal Science
, Barbara Bilińska - Jagiellonian University (UJ)
Barbara Bilińska,,
, Bartosz Płachno - Jagiellonian University (UJ)
Bartosz Płachno,,
, Zbigniew Arent (VET)
Zbigniew Arent,,
- University Centre for Veterinary Medicine UJ-UR
, Izabela Krakowska (VET)
Izabela Krakowska,,
- University Centre for Veterinary Medicine UJ-UR
, Kazimierz Tarasiuk (VET)
Kazimierz Tarasiuk,,
- University Centre for Veterinary Medicine UJ-UR
Journal seriesActa Histochemica, ISSN 0065-1281, e-ISSN 1618-0372, (N/A 70 pkt)
Issue year2020
Publication size in sheets0.55
Article number151526
Keywords in EnglishBoar,Estrogen-related receptor, G protein - coupled estrogen receptor, Peroxisome proliferator - activated receptor, Testis
ASJC Classification2700 General Medicine; 2722 Histology; 1307 Cell Biology
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.668; WoS Impact Factor: 2018 = 1.719 (2) - 2018=1.594 (5)
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