Changes in heart morphometric parameters over the course of a monocrotaline-induced pulmonary arterial hypertension rat model

Mateusz K. Hołda , Elżbieta Szczepanek , Joanna Bielawska , Natalia Palka , Dorota Wojtysiak , Paulina Frączek , Michał Nowakowski , Natalia Sowińska , Zbigniew Arent , Piotr Podolec , Grzegorz Kopeć

Abstract

Background: Aim of this study was to assess changes in cardiac morphometric parameters at diferent stages of pulmonary arterial hypertension (PAH) using a monocrotaline-induced rat model. Methods: Four groups were distinguished: I–control, non-PAH (n=18); II–early PAH (n=12); III–end-stage PAH (n=23); and IV–end-stage PAH with myocarditis (n=7). Results: Performed over the course of PAH in vivo echocardiography showed signifcant thickening of the right ventricle free wall (end-diastolic dimension), tricuspid annular plane systolic excursion reduction and decrease in pulmonary artery acceleration time normalized to cycle length. No diferences in end-diastolic left ventricle free wall thickness measured in echocardiography was observed between groups. Signifcant increase of right ventricle and decrease of left ventricle systolic pressure was observed over the development of PAH. Thickening and weight increase (241.2% increase) of the right ventricle free wall and signifcant dilatation of the right ventricle was observed over the course of PAH (p<0.001). Reduction in the left ventricle free wall thickness was also observed in end-stage PAH (p<0.001). Signifcant trend in the left ventricle free wall weight decrease was observed over the course of PAH (p<0.001, 24.3% reduction). Calculated right/left ventricle free wall weight ratio gradually increased over PAH stages (p<0.001). The reduction of left ventricle diameter was observed in rats with end-stage PAH both with and without myocarditis (p<0.001). Conclusions: PAH leads to multidimensional changes in morphometric cardiac parameters. Right ventricle morphological and functional failure develop gradually from early stage of PAH, while left ventricle changes develop at the end stages of PAH.
Author Mateusz K. Hołda - Jagiellonian University (UJ)
Mateusz K. Hołda,,
-
, Elżbieta Szczepanek - Jagiellonian University (UJ)
Elżbieta Szczepanek,,
-
, Joanna Bielawska - Manor Veterinary Clinic, Folkestone, UK
Joanna Bielawska,,
-
, Natalia Palka - Jagiellonian University (UJ)
Natalia Palka,,
-
, Dorota Wojtysiak (FoAS / Department of Genetics, Animal Breeding and Ethology)
Dorota Wojtysiak,,
- Department of Genetics, Animal Breeding and Ethology
, Paulina Frączek - The University Hospital in Cracow
Paulina Frączek,,
-
, Michał Nowakowski (VET)
Michał Nowakowski,,
- University Centre for Veterinary Medicine UJ-UR
, Natalia Sowińska (VET)
Natalia Sowińska,,
- University Centre for Veterinary Medicine UJ-UR
, Zbigniew Arent (VET)
Zbigniew Arent,,
- University Centre for Veterinary Medicine UJ-UR
, Piotr Podolec - Jagiellonian University (UJ)
Piotr Podolec,,
-
et al.`
Journal seriesJournal of Translational Medicine, ISSN 1479-5876, (N/A 100 pkt)
Issue year2020
No18
Pages1-11
Publication size in sheets0.5
Article number262
Keywords in EnglishPulmonary hypertension, Monocrotaline-induced PAH, Cardiac remodelling, Left ventricle mass loss, Right ventricular failure
ASJC Classification2700 General Medicine; 1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.1186/s12967-020-02440-7
URL https://translational-medicine.biomedcentral.com/track/pdf/10.1186/s12967-020-02440-7
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
Changes in heart morphometric parameters over the course of a monocrotaline-induced pulmonary arterial hypertension rat model of 16-07-2020
1,68 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.133; WoS Impact Factor: 2018 = 4.098 (2) - 2018=4.301 (5)
Citation count*
Additional fields
FinansowanieThis study was funded by the Ministry of Science and Higher Education, Republic of Poland from budgetary funds for science (2016–2018) as a research project under the “Diamond Grant” programme (No. DI2015 011145; 0111/DIA/2016/45). The funding agencies have not the influence on the design of the study and collection, analysis, and interpretation of data and on writing the manuscript.
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?