Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age

T. Ząbek , E. Semik-Gurgul , Tomasz Szmatoła , A. Gurgul , A. Fornal , Monika Bugno-Poniewierska

Abstract

Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.
Author T. Ząbek
T. Ząbek,,
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, E. Semik-Gurgul
E. Semik-Gurgul,,
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, Tomasz Szmatoła (VET)
Tomasz Szmatoła,,
- University Centre for Veterinary Medicine UJ-UR
, A. Gurgul
A. Gurgul,,
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, A. Fornal
A. Fornal,,
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, Monika Bugno-Poniewierska (FoAS / IoVS)
Monika Bugno-Poniewierska,,
- Institute of Veterinary Sciences
Journal seriesInternational Journal of Genomics, ISSN 2314-436X, e-ISSN 2314-4378, (N/A 70 pkt)
Issue year2019
Vol2019
Pages1-9
Publication size in sheets0.5
Article number2839614
ASJC Classification1303 Biochemistry; 1311 Genetics; 1312 Molecular Biology; 3003 Pharmaceutical Science
DOIDOI:10.1155/2019/2839614
URL http://downloads.hindawi.com/journals/ijg/2019/2839614.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.558; WoS Impact Factor: 2018 = 2.303 (2) - 2018=2.475 (5)
Citation count*
Additional fields
FinansowanieThe research was funded by the statutory research project (04-18-14-21) of the National Research Institute of Animal Production (Poland).
OświadczenieT. Szmatoła
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