Short communication: Interrelationship between butyrate and glucose supply on butyrate and glucose oxidation by ruminal epithelial preparations

B. I. Wise , Paweł Górka , T. Mutsvangwa , E. Okine , G. B. Penner

Abstract

The aim of this study was to determine whether dietary Na-butyrate supplementation affects butyrate and glucose oxidation by ruminal epithelial preparations and whether this effect can be acutely modulated by substrate (glucose and butyrate) supply. Eighteen Suffolk wether lambs (6 lambs/treatment) were blocked by body weight and, within block, randomly assigned to the control treatment (CON) or to diets containing differing Na-butyrate inclusion rates (1.58 or 3.16%) equating to 1.25 (B1.25), and 2.50% (B2.50) butyrate on a dry matter basis, respectively. All lambs received their diet for a period of 14 d. After dietary adaptation, lambs were killed and the ruminal epithelium was harvested from the ventral sac, minced finely, and used for in vitro incubations. Incubation medium contained either a constant concentration of glucose (4 mM) with increasing butyrate concentrations (0, 5, 15, 25, or 40 mM) or a constant butyrate concentration (15 mM) with increasing glucose concentrations (0, 1, 2, 4, or 8 mM) to allow for the evaluation of whether acute changes in the concentration of metabolic substrates affect the oxidation of glucose and butyrate. We observed no interactions between the in vivo and in vitro treatments. Increasing dietary butyrate supplementation linearly decreased glucose oxidation by ruminal epithelial preparations, but had no effect on butyrate oxidation. Increasing butyrate concentration in vitro decreased (cubic effect) glucose oxidation when butyrate concentration ranged between 5 and 15 mM; however, glucose oxidation was increased with a butyrate concentration of 40 mM. Butyrate oxidation decreased (cubic effect) as glucose concentration increased from 1 to 4 mM; however, butyrate oxidation increased when glucose was included at 8 mM. The results of this study demonstrate that dietary butyrate supplementation can decrease glucose oxidation by the ruminal epithelium, but the relative supply of glucose and butyrate has a pronounced effect on substrate oxidation.
Author B. I. Wise
B. I. Wise,,
-
, Paweł Górka (DoAS / DoANaFS)
Paweł Górka,,
- Department of Animal Nutrition and Feed Science
, T. Mutsvangwa
T. Mutsvangwa,,
-
, E. Okine
E. Okine,,
-
, G. B. Penner
G. B. Penner,,
-
Journal seriesJournal of Dairy Science, ISSN 0022-0302, (A 45 pkt)
Issue year2013
Vol96
No9
Pages5914-5918
Publication size in sheets0.5
Keywords in Englishbutyrate, glucose, ruminal epithelium, oxidation
ASJC Classification1311 Genetics; 1103 Animal Science and Zoology; 1106 Food Science
DOIDOI:10.3168/jds.2013-6677
URL http://www.sciencedirect.com/science/article/pii/S0022030213004621
Internal identifierWHiBZ/2013/67
Languageen angielski
Score (nominal)45
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.589; WoS Impact Factor: 2013 = 2.55 (2) - 2013=3.08 (5)
Citation count*
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FinansowanieFunding for the project was provided by the Presidents NSERC Fund at the University of Saskatchewan. Stipend support for B. I. Wiese was provided from the NSERC-USRA program. The authors thank D. J. Wilson (University of Saskatchewan, Saskatoon, SK, Canada) for help with animal care and sample collection.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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