Application of QuEChERS-EMR-Lipid-DLLME method for the determination of polycyclic aromatic hydrocarbons in smoked food of animal origin

Teresa Slámová , Anna Sadowska-Rociek , Adéla Fraňková , Magdalena Surma , Jan Banout


The aim of this study was to develop an effective sample preparation procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in smoked fatty products of animal origin (fish, cheese and sausage). Two different approaches were tested: classical QuEChERS and procedure with the use of Enhanced Matrix Removal (EMR)-Lipid material. Two techniques of extract preconcentration: under nitrogen stream and with the use of dispersive liquid-liquid microextraction (DLLME), were also taken into consideration. All samples were analysed using gas chromatography-mass spectrometry. The results showed the optimised sample preparation procedure was composed of three steps: 1) QuEChERS extraction, 2) clean-up by EMR-Lipid material and 3) extract preconcentration by DLLME. The obtained recovery rates within the range of 50–120% were received for all compounds with relative standard deviation (RSD) values lower than 16.7%. The proposed method is fast and effective and can be successfully applied for PAHs determination in difficult matrices such as heat-treated food of animal origin with high fat content. The research also discovered the significance of the quality of the laboratory disposables. Contaminants present in plastic consumables can be transferred to the sample extract contributing to its contamination and can also lead to failure of analytical equipment.
Author Teresa Slámová
Teresa Slámová,,
, Anna Sadowska-Rociek (FoFT / DoToCaC)
Anna Sadowska-Rociek,,
- Department of Technology of Catering and Consumption
, Adéla Fraňková
Adéla Fraňková,,
, Magdalena Surma (FoFT / DoToCaC)
Magdalena Surma,,
- Department of Technology of Catering and Consumption
, Jan Banout
Jan Banout,,
Journal seriesJournal of Food Composition and Analysis, ISSN 0889-1575, e-ISSN 1096-0481, (N/A 100 pkt)
Issue year2020
Publication size in sheets0.5
Article number103420
Keywords in EnglishFood analysis, Food composition, Polycyclic aromatic hydrocarbons, Smoked food, QuEChERS, EMR-Lipid
ASJC Classification1106 Food Science
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.46; WoS Impact Factor: 2018 = 2.994 (2) - 2018=3.44 (5)
Citation count*
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FinansowanieThis research was performed with the financial support from Ministry of Science and Higher Education of Republic of Poland within the statutory R & D activities (DS-3700/KTGiK/2018), and by Internal Grant Agency of Faculty of Tropical AgriSciences: grant number 20195006.
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