The use of PCR-RFLP technique for the analysis of yeast contaminants in winemaking

Paweł Satora , Iwona Drożdż , Aleksandra Duda-Chodak , Katarzyna Skrzypiec

Abstract

During the production of wine may occur infections of microbial, which can negatively affects the quality and characteristics sensory of product. In order to control the quality of given product, molecular techniques are used. They allow to quick and accurate identify of spoilage microorganism. PCR-RFLP method is very useful for study of the yeasts.It is based on amplification of rDNA gene repeat unit, which includes 5,8 rRNA gene and two non-coding internal transcribed spacers ITS1 and ITS2. The PCR products are then subjected to restriction enzyme digestion and electrophoretic separation. During experiments used HaeIII enzyme was also used and identification of yeast strains was made by comparing the length of restriction patterns in agarose gel. This allowed for quick and easy classification of the studied microorganisms. Distinguish between strains of the same species with a single restriction enzyme, proved to be virtually impossible, because of the similar length of the restriction fragments.
Author Paweł Satora (FoFT / DoFTaTM)
Paweł Satora,,
- Department of Fermentation Technology and Technical Microbiology
, Iwona Drożdż (FoFT / DoFTaTM)
Iwona Drożdż,,
- Department of Fermentation Technology and Technical Microbiology
, Aleksandra Duda-Chodak (FoFT / DoFTaTM)
Aleksandra Duda-Chodak,,
- Department of Fermentation Technology and Technical Microbiology
, Katarzyna Skrzypiec (FoFT / DoFTaTM)
Katarzyna Skrzypiec,,
- Department of Fermentation Technology and Technical Microbiology
Journal seriesPotravinárstvo, ISSN 1338-0230, (B 5 pkt)
Issue year2013
Vol7
NoSpecial Issue
Pages16-19
Publication size in sheets0.5
Keywords in English5.8-ITS region, microbiological c ontamination, PCR-RFLP, yeasts
ASJC Classification1106 Food Science
URL http://www.potravinarstvo.com/dokumenty/mc_march_2013/mikrobiologicka_bezpecnost_potravin/satora.pdf
Internal identifierWTŻ/2013/16
Languageen angielski
Not used for evaluationyes
Score (nominal)0
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2014 = 0.037
Citation count*1 (2020-04-02)
Additional fields
OświadczeniePaweł Satora, Iwona Drożdż, Aleksandra Duda-Chodak, Katarzyna Skrzypiec
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