Preliminary evaluation of application of a 3-dimensional network structure of siloxanes Dergall preparation on chick embryo development and microbiological status of eggshells

Michał Patrzałek , Maja Kosecka-Strojek , Klaudia Lisowska-Łysiak , Magdalena Trela , Mariusz Kot , Magdalena Gawlak , Dawid Liszka , Mieczysław Sajewicz , Barbara Tombarkiewicz , Krzysztof Pawlak , Jacek Międzobrodzki , Marcin W. Lis

Abstract

The spatial network structure of Dergall is based on substances nontoxic to humans and the environment which, when applied on solid surfaces, creates a coating that reduces bacterial cell adhesion. The bacteriostatic properties of siloxanes are based on a purely physical action mechanism which excludes development of drug-resistant microorganisms. The aims of the present study were to 1) evaluate a Dergall layer formed on the eggshell surface regarding the potential harmful effects on the chick embryo; 2) evaluate antimicrobial activity and estimate the prolongation time of Dergall's potential antimicrobial activity. Dergall at a concentration of 0.6% formed a layer on the eggshell surface. In vitro testing of the potential harmful effects of Dergall by means of a hen embryo test of the chorioallantoic membrane showed no irritation reaction at a concentration of 3% and lower. The hatchability of the groups sprayed with a Dergall water solution with a concentration of 0 to 5% was 89.1 to 93.8% for fertilized eggs (P > 0.05) but decreased to 63.7% (P < 0.05) in the group sprayed with a 6% concentration of the solution. This phenomenon was caused by embryo mortality in the first week of incubation. At the concentration of 0.6%, Dergall exhibited strong antibacterial properties against bacteria such as Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Shigella flexneri, and Salmonella typhimurium. For Streptococcus pyogenes, the highest antibacterial activity of Dergall was reported in the concentrations of 100 and 50%. For Pseudomonas aeruginosa, no antibacterial activity of Dergall was generally observed, but in vivo testing showed a strong decrease of all gram-negative bacteria growth. Moreover, a prolonged antimicrobial effect lasting until 3 D after disinfection was observed, which makes Dergall a safe and efficient disinfectant.
Author Michał Patrzałek
Michał Patrzałek,,
-
, Maja Kosecka-Strojek
Maja Kosecka-Strojek,,
-
, Klaudia Lisowska-Łysiak
Klaudia Lisowska-Łysiak,,
-
, Magdalena Trela (FoAS / IoVS)
Magdalena Trela,,
- Institute of Veterinary Sciences
, Mariusz Kot
Mariusz Kot,,
-
, Magdalena Gawlak
Magdalena Gawlak,,
-
, Dawid Liszka
Dawid Liszka,,
-
, Mieczysław Sajewicz
Mieczysław Sajewicz,,
-
, Barbara Tombarkiewicz (FoAS / IoVS)
Barbara Tombarkiewicz,,
- Institute of Veterinary Sciences
, Krzysztof Pawlak (FoAS / IoVS)
Krzysztof Pawlak,,
- Institute of Veterinary Sciences
et al.`
Journal seriesPoultry Science, ISSN 0032-5791, e-ISSN 1525-3171, (N/A 140 pkt)
Issue year2020
Vol99
No3
Pages1581-1590
Publication size in sheets0.5
Keywords in Englishsilicon, antibacterial effect, HET-CAM, chicken
ASJC Classification2700 General Medicine; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.psj.2019.10.079
URL https://www.sciencedirect.com/science/article/pii/S0032579119578051/pdfft?md5=851db45910cc65657b71007a105334ec&pid=1-s2.0-S0032579119578051-main.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
Additional file
Preliminary evaluation of application of a 3-dimensional network structure of siloxanes Dergall preparation on chick embryo development and microbiological status of eggshells of 06-03-2020
1,32 MB
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.38; WoS Impact Factor: 2018 = 2.027 (2) - 2018=2.537 (5)
Citation count*
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FinansowanieThe research project was funded by ICB Pharma, Jaworzno, Poland. The faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University is a part of the Leading National Research Centre (KNOW) which is supported by the Ministry of Science and Higher Education of the Republic of Poland.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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