Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows
Joanna Pokorska , Dominika Kułaj , Agata Piestrzyńska-Kajtoch , Anna Radko
AbstractIn the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.
|Journal series||Animals, ISSN 2076-2615, e-ISSN 2076-2615, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||antioxidant activity; milk; lipocalin-2; gene polymorphism|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 0; : 2018 = 1.148; : 2018 = 1.832 (2)|
|Finansowanie||The authors wish to acknowledge financial support from the Ministry of Science and Higher Education in Poland (DS 3259/2018).|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.