Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Joanna Pokorska , Dominika Kułaj , Agata Piestrzyńska-Kajtoch , Anna Radko

Abstract

In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.
Author Joanna Pokorska (FoAS / IoAS)
Joanna Pokorska,,
- Institute of Animal Science
, Dominika Kułaj (FoAS / IoAS)
Dominika Kułaj,,
- Institute of Animal Science
, Agata Piestrzyńska-Kajtoch
Agata Piestrzyńska-Kajtoch,,
-
, Anna Radko
Anna Radko,,
-
Journal seriesAnimals, ISSN 2076-2615, e-ISSN 2076-2615, (N/A 100 pkt)
Issue year2019
Vol9
No11
Pages1-9
Publication size in sheets0.5
Article number992
Keywords in Englishantioxidant activity; milk; lipocalin-2; gene polymorphism
ASJC Classification3400 General Veterinary; 1103 Animal Science and Zoology
DOIDOI:10.3390/ani9110992
URL https://www.mdpi.com/2076-2615/9/11/992/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows of 20-11-2019
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Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.148; WoS Impact Factor: 2018 = 1.832 (2)
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FinansowanieThe authors wish to acknowledge financial support from the Ministry of Science and Higher Education in Poland (DS 3259/2018).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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