Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation

Aneta Koronowicz , Paula Banks , Dominik Domagała , Adam Master , Teresa Leszczyńska , Ewelina Piasna , Mariola Marynowska , Piotr Laidler

Abstract

BACKGROUND: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology. RESULTS: We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes. CONCLUSIONS: We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens' diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent.
Author Aneta Koronowicz (FoFT / DoHN)
Aneta Koronowicz,,
- Department of Human Nutrition
, Paula Banks (FoFT / DoHN)
Paula Banks,,
- Department of Human Nutrition
, Dominik Domagała (FoFT / DoHN)
Dominik Domagała,,
- Department of Human Nutrition
, Adam Master
Adam Master,,
-
, Teresa Leszczyńska (FoFT / DoHN)
Teresa Leszczyńska,,
- Department of Human Nutrition
, Ewelina Piasna (FoFT / DoHN)
Ewelina Piasna,,
- Department of Human Nutrition
, Mariola Marynowska (FoFT / DoHN)
Mariola Marynowska,,
- Department of Human Nutrition
, Piotr Laidler
Piotr Laidler,,
-
Journal seriesGenes and Nutrition, ISSN 1555-8932, (A 30 pkt)
Issue year2016
Vol11
No22
Pages1-12
Publication size in sheets0.55
Keywords in EnglishAKT/mTOR pathway, CLA-enriched egg yolks, Cancer chemoprevention, DNA microarray, MCF-7 cancer cells, SFA/MUFA, Transcriptome
DOIDOI:10.1186/s12263-016-0537-z
URL https://genesandnutrition.biomedcentral.com/track/pdf/10.1186/s12263-016-0537-z
Internal identifierWTŻ/2016/124
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)30
ScoreMinisterial score = 30.0, 26-07-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 26-07-2017, ArticleFromJournal
Publication indicators WoS Citations = 5
Citation count*
Additional fields
FinansowanieThis work was supported by the Polish National Science Center (grant number 2011/03/B/NZ9/01423) “Conjugated linoleic acid (CLA)-induced transcriptional activation of PPAR-an investigation of molecular mechanisms of putative anticancer action of fatty acids of CLA-enriched egg yolks.”
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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