DcSto: carrot Stowaway-like elements are abundant, diverse, and polymorphic

Alicja Macko-Podgórni , Anna Nowicka , Ewa Grzebelus , Philipp W. Simon , Dariusz Grzebelus

Abstract

We investigated nine families of Stowaway-like miniature inverted-repeat transposable elements (MITEs) in the carrot genome, named DcSto1 to DcSto9. All of them were AT-rich and shared a highly conserved 6 bp-long TIR typical for Stowaways. The copy number of DcSto1 elements was estimated as ca. 5,000 per diploid genome. We observed preference for clustered insertions of DcSto and other MITEs. Distribution of DcSto1 hybridization signals revealed presence of DcSto1 clusters within euchromatic regions along all chromosomes. An arrangement of eight regions encompassing DcSto insertion sites, studied in detail, was highly variable among plants representing different populations of Daucus carota. All of these insertions were polymorphic which most likely suggests a very recent mobilization of those elements. Insertions of DcSto near carrot genes and presence of putative promoters, regulatory motifs, and polyA signals within their sequences might suggest a possible involvement of DcSto in the regulation of gene expression.
Author Alicja Macko-Podgórni (FoH / DoGPBaSS)
Alicja Macko-Podgórni,,
- Department of Genetics, Plant Breeding and Seed Science
, Anna Nowicka (FoH / DoGPBaSS)
Anna Nowicka,,
- Department of Genetics, Plant Breeding and Seed Science
, Ewa Grzebelus (FoH / DoGPBaSS)
Ewa Grzebelus,,
- Department of Genetics, Plant Breeding and Seed Science
, Philipp W. Simon
Philipp W. Simon,,
-
, Dariusz Grzebelus (FoH / DoGPBaSS)
Dariusz Grzebelus,,
- Department of Genetics, Plant Breeding and Seed Science
Journal seriesGenetica, ISSN 0016-6707, (A 20 pkt)
Issue year2013
Vol141
No4
Pages255-267
Publication size in sheets0.6
Keywords in Englishtransposable elements, MITE, Stowaway, carrot, polymorphism
ASJC Classification1109 Insect Science; 1110 Plant Science; 1311 Genetics; 1103 Animal Science and Zoology; 2700 General Medicine
DOIDOI:10.1007/s10709-013-9725-6
URL http://link.springer.com/article/10.1007%2Fs10709-013-9725-6
Internal identifierWBIO/2013/110
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 9; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.726; WoS Impact Factor: 2013 = 1.746 (2) - 2013=2.124 (5)
Citation count*3 (2016-06-29)
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