The effect of genotype on a barley scutella culture. Histological aspects

Halina Ślesak , Grzegorz Góralski , Helena Pawłowska , Barbara Skucińska , Marzena Popielarska-Konieczna , Andrzej J. Joachimiak


Cereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).
Author Halina Ślesak
Halina Ślesak,,
, Grzegorz Góralski
Grzegorz Góralski,,
, Helena Pawłowska (FoAE / DoPBaS)
Helena Pawłowska,,
- Department of Plant Breeding and Seed
, Barbara Skucińska (FoAE / DoPBaS)
Barbara Skucińska,,
- Department of Plant Breeding and Seed Science
, Marzena Popielarska-Konieczna
Marzena Popielarska-Konieczna,,
, Andrzej J. Joachimiak
Andrzej J. Joachimiak,,
Journal seriesCentral European Journal of Biology, ISSN 1895-104X, [1644-3632 ], (A 20 pkt)
Issue year2013
Publication size in sheets0.5
Keywords in EnglishMicropropagation, Organogenesis, Somatic embryogenesis, Hordeum vulgare, Plant tissue culture
ASJC Classification1100 General Agricultural and Biological Sciences; 2400 General Immunology and Microbiology; 1300 General Biochemistry, Genetics and Molecular Biology; 2800 General Neuroscience
Internal identifierWRE/2013/24
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Impact Factor: 2013 = 0.633 (2) - 2013=0.748 (5)
Citation count*2 (2016-02-17)
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