Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum L. plants cultured in RITA® bioreactor

Agata Ptak , Emilia Morańska , Edyta Skrzypek , Marzena Warchoł , Rosella Spina , Dominique Laurain-Mattar , Magdalena Simlat

Abstract

Background: Leucojum aestivum L. is an important medicinal plant which produces Amaryllidaceaealkaloids,especiallygalanthamineandlycorine.Researchiscurrently exploring the possibility of producing these alkaloids using biotechnological methods,including invitrocultures.The biosynthesisof alkaloidsmay beaffectedby the types and concentrations of carbohydrate sources used in the medium. In the present investigation we performed such studies on in vitro cultures of L. aestivum with a view to obtaining plant material of good quality, characterized, in particular, by a high content of valuable Amaryllidaceae alkaloids. Methods: We examined the effects of various types of carbohydrate sources— sucrose, glucose, fructose and maltose—at different concentrations (30, 60 and 90 g/L)—on the quality of L. aestivum plants grown in the RITA® bioreactor. The plants’ quality was assessed by their biomass increments, as well by as analysing photosynthetic pigments, endogenous sugar, phenolics and Amaryllidaceae alkaloid content. We also investigated the effect of sugars on the activity of the antioxidant enzymes catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Results: The highest biomass increments were observed in plants cultivated in the medium containing 90 g/L sucrose. The highest CAT activity was noted in cultures growing in the medium supplemented with 90 g/L maltose, while the highest POD activity was observed in the presence of 90 g/L fructose and 60 g/L maltose. No differences in SOD activity were observed. Moreover, the sugars did not affect the contents of chlorophyll a and carotenoids, whereas the highest amount of chlorophyll b was recorded in plants growing in the medium with 60 g/L maltose. No statistically significant differences were observed in the contents of endogenous sugars and phenolicsin anyin vitroconditions. However,the additionof sugar hada decisive effect on the biosynthesis of the Amaryllidaceae alkaloids. The highest distributionofalkaloidsoccurredinplantsculturedinthemediumcontaining60g/L sucrose. Six Amaryllidaceae alkaloids were detected in the plant tissue. The addition of 30 g/L fructose in the medium resulted in the accumulation of five alkaloids, including ismine, which was not identified in other analysed tissues. The highest concentration of galanthamine was observed in plants cultured in the presence of 30 g/L fructose and 60 g/L sucrose (39.2 and 37.5 µg/g of dry weight (DW), respectively). The plants grown in the medium containing 60 g/L sucrose exhibited the highest lycorine content (1048 µg/g of DW). Conclusions: The type and concentration of sugar used in the medium have an essential influence on the biosynthesis of Amaryllidaceae alkaloids in L. aestivum plants cultured in a RITA® bioreactor. The results point to an interesting approach for commercial production of galanthamine and lycorine.
Author Agata Ptak (FoAaE / DoPoPBaSP)
Agata Ptak,,
- Department of Physiology of Plant Breeding and Seed Production
, Emilia Morańska (FoAE / DoPBaS)
Emilia Morańska,,
- Department of Plant Breeding and Seed Science
, Edyta Skrzypek - The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences
Edyta Skrzypek,,
-
, Marzena Warchoł - The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences
Marzena Warchoł,,
-
, Rosella Spina - University of Lorraine
Rosella Spina,,
-
, Dominique Laurain-Mattar - University of Lorraine
Dominique Laurain-Mattar,,
-
, Magdalena Simlat (FoAaE / DoPoPBaSP)
Magdalena Simlat,,
- Department of Physiology of Plant Breeding and Seed Production
Journal seriesPeerJ, ISSN 2167-8359, (N/A 100 pkt)
Issue year2020
No8:e8688
Pages1-19
Publication size in sheets0.9
Keywords in EnglishLeucojum aestivum, In vitro, Carbon sources, Galanthamine, Lycorine
ASJC Classification2700 General Medicine; 1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology; 2800 General Neuroscience
DOIDOI:10.7717/peerj.8688
URL https://peerj.com/articles/8688.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum L. plants cultured in RITA® bioreactor of 27-03-2020
3,96 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.865; WoS Impact Factor: 2018 = 2.353 (2) - 2018=2.7 (5)
Citation count*
Additional fields
FinansowanieThe project was financed from the subsidy of the Ministry of Science and Higher Education of the Republic of Poland awarded to the University of Agriculture in Krakow, Poland in 2019 year. This research was also supported by the MEAE and MESRI PHC Polonium project (DWM.WKE.183.10.2018/AM, 2018–2019) financed by the Ministry of Science and Higher Education of the Republic of Poland and by Institute Jean Barriol (IJB), France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?