Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor

Dorota Zięba-Przybylska , Weronika Biernat , Małgorzata Szczęsna , Katarzyna Kirsz , Tomasz Misztal

Abstract

We hypothesized that resistin is engaged in the development of leptin central insensitivity/resistance in sheep, which is a unique animal model to explore reversible leptin resistance. Thirty Polish Longwool ewes, which were ovariectomized with estrogen replacement, were used. Treatments consisted of the intravenous injection of control (saline) or recombinant bovine resistin (rbresistin): control (Control; n = 10), a low dose of rbresistin (R1; 1.0 μg/kg body weight (BW); n = 10), and a high dose of rbresistin (R2; 10.0 μg/kg BW; n = 10). The studies were performed during short-day (SD) and long-day (LD) photoperiods. Leptin and resistin concentrations were determined. Expression levels of a suppressor of cytokine signaling (SOCS)-3 and the long form of the leptin receptor (LeptRb) were determined in selected brain regions, including in the anterior pituitary (AP), hypothalamic arcuate nucleus (ARC), preoptic area (POA), and ventro- and dorsomedial nuclei (VMH/DMH). The results indicate that resistin induced a consistent decrease in LeptRb (except in POA) and an increase in SOCS-3 expression during the LD photoperiod in all selected brain regions. In conclusion, the results demonstrate that the action of resistin appears to be strongly associated with photoperiod-driven changes in the leptin signaling pathway, which may underlie the phenomenon of central leptin resistance.
Author Dorota Zięba-Przybylska (FoAS / DoAB)
Dorota Zięba-Przybylska,,
- Department of Animal Biotechnology
, Weronika Biernat (FoAS / DoAB)
Weronika Biernat,,
- Department of Animal Biotechnology
, Małgorzata Szczęsna (FoAS / DoAB)
Małgorzata Szczęsna,,
- Department of Animal Biotechnology
, Katarzyna Kirsz (FoAS / DoAB)
Katarzyna Kirsz,,
- Department of Animal Biotechnology
, Tomasz Misztal
Tomasz Misztal,,
-
Journal seriesNutrients, ISSN 2072-6643, (N/A 140 pkt)
Issue year2019
Vol11
No9
Pages1-13
Publication size in sheets0.6
Keywords in Englishleptin; leptin receptor; leptin resistance; photoperiod; resistin; sheep; SOCS-3
ASJC Classification1106 Food Science
DOIDOI:10.3390/nu11092180
URL https://www.mdpi.com/2072-6643/11/9/2180/htm
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor of 18-09-2019
1,12 MB
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.392; WoS Impact Factor: 2018 = 4.171 (2) - 2018=4.813 (5)
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FinansowanieA grant obtained from the National Science Center (2015/19/B/NZ9/01314) supported this work
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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