Impact of foliar application of some metal nanoparticles on antioxidant system in oakleaf lettuce seedlings

Rita Jurkow , Robert Pokluda , Agnieszka Sękara , Andrzej Kalisz

Abstract

Background: Nanoparticles (NPs) serve various industrial and household purposes, and their increasing use creates an environmental hazard because of their uncontrolled release into ecosystems. An important aspect of the risk assessment of NPs is to understand their interactions with plants. The aim of this study was to examine the effect of Au (10 and 20 ppm), Ag, and Pt (20 and 40 ppm) NPs on oakleaf lettuce, with particular emphasis on plant antioxidative mechanisms. Nanoparticles were applied once on the leaves of 2-week-old lettuce seedlings, after next week laboratory analyses were performed. Results: The antioxidant potential of oakleaf lettuce seedlings sprayed with metal NPs at different concentrations was investigated. Chlorophylls, fresh and dry weight were also determined. Foliar exposure of the seedlings to metal NPs did not affect ascorbate peroxidase activity, total peroxidase activity increased after Au-NPs treatment, but decreased after applying Ag-NPs and Pt-NPs. Both concentrations of Au-NPs and Pt-NPs tested caused an increase in glutathione (GSH) content, while no NPs affected L-ascorbic acid content in the plants. Ag-NPs and PtNPs applied as 40 ppm solution increased total phenolics content by 17 and 15%, respectively, compared to the control. Carotenoids content increased when Ag-NPs and Au-NPs (20 and 40 ppm) and Pt-NPs (20 ppm) were applied. Plants treated with 40 ppm of Ag-NPs and Pt-NPs showed significantly higher total antioxidant capacity and higher concentration of chlorophyll a (only for Ag-NPs) than control. Pt-NPs applied as 40 ppm increased fresh weight and total dry weight of lettuce shoot. Conclusions: Results showed that the concentrations of NPs applied and various types of metal NPs had varying impact on the antioxidant status of oakleaf lettuce. Alteration of POX activity and in biosynthesis of glutathione, total phenolics, and carotenoids due to metal NPs showed that tested nanoparticles can act as stress stimuli. However, judging by the slight changes in chlorophyll concentrations and in the fresh and dry weight of the plants, and even based on the some increases in these traits after M-NPs treatment, the stress intensity was relatively low, and the plants were able to cope with its negative effects.
Author Rita Jurkow (FoBaH / Department of Horticulture)
Rita Jurkow,,
- Department of Horticulture
, Robert Pokluda - Mendel University in Brno
Robert Pokluda,,
-
, Agnieszka Sękara (FoBaH / Department of Horticulture)
Agnieszka Sękara,,
- Department of Horticulture
, Andrzej Kalisz (FoBaH / Department of Horticulture)
Andrzej Kalisz,,
- Department of Horticulture
Journal seriesBMC Plant Biology, ISSN 1471-2229, (N/A 140 pkt)
Issue year2020
Vol20
Pages1-12
Publication size in sheets0.55
Article number290
Keywords in EnglishAntioxidants, Chlorophyll, Gold, Lactuca sativa L. var. foliosa, Nanometals, Platinum, Silver
ASJC Classification1110 Plant Science
DOIDOI:10.1186/s12870-020-02490-5
URL https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-020-02490-5
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Impact of foliar application of some metal nanoparticles on antioxidant system in oakleaf lettuce seedlings of 26-06-2020
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Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.303; WoS Impact Factor: 2018 = 3.67 (2) - 2018=4.311 (5)
Citation count*
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FinansowanieThis work was financially supported by the Ministry of Science and Higher Education of the Republic of Poland. The authors declare that the funding bodies had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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