Expression and Localization of Growth Hormone Receptor in the Oviduct of the Laying hen (Gallus domesticus)

Anna Hrabia , Agnieszka K. Grzegorzewska , Andrzej Sechman

Abstract

The purpose of the present study was to examine growth hormone receptor (GHR) gene expression by real-time PCR and demonstrate immunocytochemically the localization of GHR in four chicken oviductal parts, i.e. infundibulum, magnum, isthmus and shell gland. Experiments were carried out on Hy-Line laying hens decapitated 2 h after oviposition. GHR mRNA was expressed in all examined oviductal segments with a significantly lower level in the infundibulum in comparison to other parts of the oviduct. Specific GHR immunoreactivity was also detected in the wall of the oviduct. The intensity of the staining was as follows: infundibulum < or = isthmus < or = shell gland < or = magnum. In all oviductal parts, a positive reaction for GHR was observed in the mucosa whereas a very weak or no reaction was observed in the stroma. Within the mucosa a strong reaction for GHR was observed in the epithelium of the infundibulum and in the tubular gland of the magnum, isthmus and shell gland. Immunoreactivity for GHR was very weak in the mucosal epithelium of the magnum, isthmus and shell gland. In conclusion, the results point to the possibility of an important role of GH in oviduct functions in domestic hens.
Author Anna Hrabia (DoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Agnieszka K. Grzegorzewska (DoAS / DoAPaE)
Agnieszka K. Grzegorzewska,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (DoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Journal seriesFolia Biologica-Krakow, ISSN 0015-5497, (A 20 pkt)
Issue year2013
Vol61
No3-4
Pages271-276
Publication size in sheets0.5
Keywords in Englishgrowth hormone receptor, RT-PCR, immunocytochemistry, oviduct, chicken
ASJC Classification1300 General Biochemistry, Genetics and Molecular Biology; 2700 General Medicine
DOIDOI:10.3409/fb61_3-4.271
URL http://www.isez.pan.krakow.pl/journals/folia/pdf/61(3-4)/61(3-4)_20.pdf
Internal identifierWHiBZ/2013/86
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 6; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.768; WoS Impact Factor: 2013 = 0.478 (2) - 2013=0.642 (5)
Citation count*
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FinansowanieSupported by grant no. DS-3243/KFiEZ, and in part by grant No. 2011/01/B/NZ4/03665 from National Science Centre, Poland (to A.H)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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