Changes in content of steroid regulators during cold hardening of winter wheat - Steroid physiological/biochemical activity and impact on frost tolerance

Anna Janeczko , Ewa Pociecha , Michał Dziurka , Barbara Jurczyk , Maria Libik-Konieczny , Jana Oklestkova , Ondřej Novák , Maria Pilarska , Maria Filek , Elżbieta Rudolphi-Skórska , Iwona Sadura , Agata Siwek

Abstract

The purpose of experiments was to describe the alterations of content of steroid regulators (brassinosteroids, progesterone) during cold hardening of winter wheat. Further we studied physiological and biochemical changes induced by these steroids in cold hardened winter wheat together with estimation of plant frost tolerance. The endogenous brassinosteroid content was elevated in winter wheat during cold hardening while level of progesterone was lowered. A higher content of brassinosteroids (but not progesterone) was connected to better frost tolerance of winter wheat cultivars. Plant supplementation with brassinosteroid (24-epibrassinolide) and progesterone before cold hardening reduced frost damage. Tests with the inhibitors of the biosynthesis of brassinosteroids and progesterone suggested that these steroids are one of players in regulating the antioxidant system in winter wheat during cold hardening. Their role in regulating the expression of Rubisco or the Rubisco activase gene was less clear. Steroid regulators did not affect the content of the stress hormone ABA. Model studies of the membranes, made on a Langmuir bath, showed an increase in the value of the parameter describing differences in membrane compressibility (resulting from stronger interactions among the molecules in the monolayers). This suggests that 24-epibrassinolide and progesterone enter into the lipid layer and - in a similar way to sterols – stabilise the interaction among lipids. It may be significant step for better frost tolerance. The use of steroid regulators (especially brassinosteroids) as agrochemicals improving frost tolerance of winter cereals will be discussed.
Author Anna Janeczko
Anna Janeczko,,
-
, Ewa Pociecha (FoAaE / DoPP)
Ewa Pociecha,,
- Department of Plant Physiology
, Michał Dziurka
Michał Dziurka,,
-
, Barbara Jurczyk (FoAE / DoPP)
Barbara Jurczyk,,
- Department of Plant Physiology
, Maria Libik-Konieczny
Maria Libik-Konieczny,,
-
, Jana Oklestkova
Jana Oklestkova,,
-
, Ondřej Novák
Ondřej Novák,,
-
, Maria Pilarska
Maria Pilarska,,
-
, Maria Filek
Maria Filek,,
-
, Elżbieta Rudolphi-Skórska
Elżbieta Rudolphi-Skórska,,
-
et al.`
Journal seriesPlant Physiology and Biochemistry, ISSN 0981-9428, (N/A 70 pkt)
Issue year2019
Vol39
Pages215-228
Publication size in sheets0.65
Keywords in EnglishABA, Brassinosteroids, Cold hardening, Frost, Progesterone, Rubisco, Sugars
ASJC Classification1110 Plant Science; 1311 Genetics; 1314 Physiology
DOIDOI:10.1016/j.plaphy.2019.03.020
URL https://www.sciencedirect.com/science/article/pii/S0981942819301020/pdfft?md5=d92eff380348fdb8f73fc71455371ed8&pid=1-s2.0-S0981942819301020-main.pdf
Internal identifierWRE/2019/89
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.092; WoS Impact Factor: 2018 = 3.404 (2) - 2018=3.607 (5)
Citation count*
Additional fields
FinansowanieThese studies were financed by the National Science Centre (Poland) (project 2013/09/B/NZ9/01653). UHPLC-MS/MS analyses of endogenous steroids were additionally financed by project of the Ministry of Education, Youth and Sports CR,European Regional Development Fund-Project “Plants as a tool for sustainable global development” no. CZ.02.1.01/0.0/0.0/16_019/0000827.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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