Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Katarzyna Andraszek , Dorota Banaszewska , Olga Szeleszczuk , Piotr Niedbała , Marta Kuchta-Gładysz

Abstract

Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark ‘collars’ in the distal part of the head. These ‘collars’ are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post‐acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique.
Author Katarzyna Andraszek - Siedlce University of Natural Sciences and Humanities
Katarzyna Andraszek,,
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, Dorota Banaszewska - Siedlce University of Natural Sciences and Humanities
Dorota Banaszewska,,
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, Olga Szeleszczuk (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Olga Szeleszczuk,,
- Department of Animal Reproduction, Anatomy and Genomics
, Piotr Niedbała (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Piotr Niedbała,,
- Department of Animal Reproduction, Anatomy and Genomics
, Marta Kuchta-Gładysz (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Marta Kuchta-Gładysz,,
- Department of Animal Reproduction, Anatomy and Genomics
Journal seriesReproduction in Domestic Animals, ISSN 0936-6768, e-ISSN 1439-0531, (N/A 100 pkt)
Issue year2020
Vol55
No4
Pages515-522
Publication size in sheets0.5
Keywords in Englishchinchilla, silver nitrate, sperm structure
ASJC Classification1103 Animal Science and Zoology; 1305 Biotechnology; 1310 Endocrinology
DOIDOI:10.1111/rda.13646
URL https://onlinelibrary.wiley.com/doi/epdf/10.1111/rda.13646
Languageen angielski
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Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis of 02-06-2020
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Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.934; WoS Impact Factor: 2018 = 1.638 (2) - 2018=1.677 (5)
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FinansowanieSiedlce University of Natural Sciences and Humanities
LicencjaFull access na stronie czasopisma 2.06.2020
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