Intra-population genetic diversity of cultivated carrot (Daucus carota L.) assessed by analysis of microsatellite markers
Anna Maksylewicz-Kaul , Rafał Barański
AbstractIntra-population variation of 18 cultivated carrot (Daucus carota L. ssp. sativus) populations of diverse origins was evaluated using codominant microsatellite (SSR) markers. Using 27 genomic and EST-derived SSR markers, 253 alleles were identified with a mean 9.4 alleles per marker. Most of the alleles (60.5%) were rare i.e., with the frequency ≤ 0.05 while only 3.95% of alleles occurred with frequency > 0.6. EST-derived SSR markers were less polymorphic than genomic SSR markers. Differences in allele occurrence allowed 16 out of 18 populations to be assigned to either the Western or Asian carrot gene pools with high probability. Populations could be also discriminated due to the presence of private alleles (25.3% of all alleles). Most populations had excess of alleles in the homozygous state indicating their inbreeding, although heterozygous loci were common in F1 hybrids. Genetic diversity was due to allelic variation among plants within populations (62% of total variation) and between populations (38%). Accessions originating from continental Asia and Europe had more allelic variants and higher diversity than those from Japan and USA. Also, allelic richness and variability in landraces was higher than in F1 hybrids and open-pollinated cultivars.
|Journal series||Acta Biochimica Polonica, ISSN 0001-527X, (A 15 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||SSR, biodiversity, genetic structure, gene pool|
|Score|| = 15.0, 30-05-2019, ArticleFromJournal|
= 15.0, 30-05-2019, ArticleFromJournal
|Citation count*||11 (2020-06-05)|
|Finansowanie||The research was supported by the Ministry of Science and Higher Education Grant No. NN310782440.|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.