Low-temperature immobilization of water in Antarctic Turgidosculum complicatulum and in Prasiola crispa. Part I. Turgidosculum complicatulum

Magdalena Bacior , Hubert Harańczyk , P. Nowak , P. Kijak , M. Marzec , Jakub Fitas , M.A. Olech

Abstract

The studies of low-temperature immobilization of bound water in Antarctic lichenized fungus Turgidosculum complicatulum were performed using 1H NMR and DSC over a wide range of thallus hydration. 1H NMR free induction decays were decomposed into a solid component well described by the Gaussian function and two exponentially decaying components coming from a tightly bound water and from a loosely bound water fraction. 1H NMR spectra revealed one averaged mobile proton signal component. 1H NMR measurements recorded in time and in frequency domain suggest the non-cooperative bound water immobilization in T. complicatulum thallus. The threshold of the hydration level estimated by 1H NMR analysis at which the cooperative bound water freezing was detected was Δm/m0 ≈ 0.39, whereas for DSC analysis was equal to Δm/m0 = 0.375. Main ice melting estimated from DSC measurements for zero hydration level of the sample starts at tm = –(19.29 ± 1.19)°C. However, DSC melting peak shows a composed form being a superposition of the main narrow peak (presumably melting of mycobiont areas) and a broad low-temperature shoulder (presumably melting of isolated photobiont cells). DSC traces recorded after two-hour incubation of T. complicatulum thallus at –20 °C suggest much lower threshold level of hydration at which the ice formation occurs (Δm/m0 = 0.0842). Presumably it is a result of diffusion induced migration of separated water molecules to ice microcrystallites already present in thallus, but still beyond the calorimeter resolution.
Author Magdalena Bacior (FoAaE / IoSSaA)
Magdalena Bacior,,
- Institute of Soil Science and Agrophysics
, Hubert Harańczyk
Hubert Harańczyk,,
-
, P. Nowak
P. Nowak,,
-
, P. Kijak
P. Kijak,,
-
, M. Marzec
M. Marzec,,
-
, Jakub Fitas (FoPaPE / DoMEaA)
Jakub Fitas,,
- Department of Mechanical Engineering and Agrophysics
, M.A. Olech
M.A. Olech,,
-
Journal seriesColloids and Surfaces B-Biointerfaces, [Colloids and Surfaces B: Biointerfaces], ISSN 0927-7765, e-ISSN 1873-4367, (N/A 100 pkt)
Issue year2019
Vol173
Pages869-875
Publication size in sheets0.5
Keywords in EnglishFreezing resistance, Phase growth, Lichens, NMR, DSC
ASJC Classification2700 General Medicine; 1305 Biotechnology; 1505 Colloid and Surface Chemistry; 1606 Physical and Theoretical Chemistry; 3110 Surfaces and Interfaces
DOIDOI:10.1016/j.colsurfb.2018.10.059
URL https://ac.els-cdn.com/S0927776518307574/1-s2.0-S0927776518307574-main.pdf?_tid=c5b62e41-ecdf-4fdb-aa94-361d1a8d6a6c&acdnat=1548246198_c0b2c44251990f12d67f9c93ac017c9b
Internal identifierWRE/2019/1
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.042; WoS Impact Factor: 2018 = 3.973 (2) - 2018=4.18 (5)
Citation count*
Additional fields
FinansowanieThe research was carried out with the equipment purchased thanks to the financial support of the European Regional Development Fund in the framework of the Polish Innovation Economy Operational Program (contract no. POIG.02.01.00-12-023/08) and also financed by the Polish Ministry of Science and Higher Education (MNiSW, contract no. 7150/E-338/M/2015 and 7150/E-338/M/2016).
Oświadczenie o afiliacjiM. Bacior
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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