Downregulation of LRb in VMH/DMH during the second half of gestation and upregulation of SOCS-3 in ARC in late-pregnant ewes - Implications for leptin resistance

Małgorzata Szczęsna , Katarzyna Kirsz , Tomasz Misztal , Dorota Zięba-Przybylska

Abstract

To investigate factors involved in pregnancy-induced regulation of tissue sensitivity to leptin, we determined leptin concentrations and expression levels of the long form of the leptin receptor (LRb) and suppressor of cytokine signalling (SOCS)-3 in the ventro- and dorsomedial nuclei (VMH/DMH), arcuate nucleus (ARC), median eminence (ME) and anterior pituitary (AP) in 15 Polish Longwool ewes euthanized at 30, 60, 90 and 120 days of pregnancy and before gestation (n = 3 per group). Leptin concentrations increased during the first half of pregnancy, peaked on day 60, and then declined. In the VMH/DMH, LRb mRNA levels decreased from day 60 of pregnancy; in the ARC, LRb mRNA levels remained stable before and throughout pregnancy. LRb expression in the ME was lower in the first two months of pregnancy than before pregnancy (P < 0.01) and peaked at day 90. In the AP, LRb mRNA levels were higher during mid-pregnancy (P < 0.05) than before pregnancy. SOCS-3 expression in the VMH/DMH was higher throughout gestation (P < 0.05) than before pregnancy but was undetectable at day 120. SOCS-3 transcript levels were higher in the ARC (P < 0.05) in late-pregnancy (at day 120) than in non-pregnant ewes. SOCS-3 mRNA levels in the ME were lower at days 30 and 60 (P < 0.05) than at day 120 or before pregnancy. In the AP, SOCS-3 transcription was stable throughout gestation except at day 120, when it increased (P < 0.05). The changes in plasma leptin concentrations during pregnancy, hypothalamic LRb downregulation in the VMH/DMH during the second half of gestation and SOCS-3 upregulation in the ARC in late-pregnant ewes identified here may be essential components of the mechanisms driving ovine leptin insensitivity.
Author Małgorzata Szczęsna (FoAS / DoAB)
Małgorzata Szczęsna,,
- Department of Animal Biotechnology
, Katarzyna Kirsz (FoAS / DoAB)
Katarzyna Kirsz,,
- Department of Animal Biotechnology
, Tomasz Misztal
Tomasz Misztal,,
-
, Dorota Zięba-Przybylska (FoAS / DoAB)
Dorota Zięba-Przybylska,,
- Department of Animal Biotechnology
Journal seriesGeneral and Comparative Endocrinology, ISSN 0016-6480, e-ISSN 1095-6840, (N/A 100 pkt)
Issue year2019
No274
Pages73-79
Publication size in sheets0.5
Keywords in EnglishLeptin, Leptin resistance, SOCS-3, Pregnancy, Sheep
ASJC Classification1310 Endocrinology
DOIDOI:10.1016/j.ygcen.2019.01.003
URL https://www.sciencedirect.com/science/article/pii/S0016648018304684
Internal identifierWHiBZ/2019/8
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.006; WoS Impact Factor: 2018 = 2.445 (2) - 2018=2.66 (5)
Citation count*
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FinansowanieThis work was supported by a grant from the National Science Centre in Poland (NCN 2013/09/B/NZ4/01532).
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