Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland

Anna Lenart , Agnieszka Klimek-Kopyra , Piotr Boroń

Abstract

The average amount of precipitation in spring and summer 2010 and 2011 coupled with relatively high temperatures caused massive Fusarium spp. infection of maize and yield losses in southern Poland. In order to examine the cause of this disease outbreak, Fusarium spp. were isolated and fungal strains were identified based on morphological characters and species-specific PCR assays. A total of 200 maize samples were processed, resulting in the obtention of 71 strains, which belonged to five Fusarium species, F. poae being the predominant one (74.56%). Other isolates were identified as F. graminearum, F. oxysporum, F. verticillioides and F. proliferatum. PCR-based detection of mycotoxin-synthesis-pathway genes was also used to determine the potential of the analyzed strains to produce trichothecenes (DON and NIV) and fumonisins (FUM). Only 14 isolates revealed the potential to produce DON (11 strains) and FUM (3 strains). HPLC analyses of grain samples revealed the presence of DON only – other mycotoxins were not detected. Moreover, 57.1% of potentially mycotoxin-producing isolates indicated the toxicity in a biological test.
Author Anna Lenart (FoAE / DoM)
Anna Lenart,,
- Department of Microbiology
, Agnieszka Klimek-Kopyra (FoAE / IoPP)
Agnieszka Klimek-Kopyra,,
- Institute of Plant Production
, Piotr Boroń (FoF / DoFP)
Piotr Boroń,,
- Department of Forest Pathology
Journal seriesPhytoparasitica, ISSN 0334-2123, (A 20 pkt)
Issue year2013
Vol41
No3
Pages241-248
Publication size in sheets0.5
Keywords in Englishmycotoxins, species-specific PCR, Zea mays L.
ASJC Classification1109 Insect Science; 1110 Plant Science
DOIDOI:10.1007/s12600-012-0284-7
URL http://link.springer.com/article/10.1007%2Fs12600-012-0284-7
Internal identifierWRE/2013/305
Languageen angielski
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 5; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.749; WoS Impact Factor: 2013 = 0.675 (2) - 2013=0.872 (5)
Citation count*2 (2016-07-31)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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