Downregulation of three novel candidate genes is important for freezing tolerance of field and laboratory cold acclimated barley

Anna Fiust , Marcin Rapacz

Abstract

Diversity arrays technology (DArT) marker sequences for barley were used for identifying new potential candidate genes for freezing tolerance (FT). We used quantitative trait loci (QTL) genetic linkage maps for FT and photosynthetic acclimation to cold for six- and two-row barley populations, and a set of 20 DArT markers obtained using the association mapping of parameters for photosynthetic acclimation to low temperatures in barley for the bioinformatics analyses. Several nucleotide and amino acid sequence, annotation databases and associated algorithms were used to identify the similarities of six of the marker sequences to potential genes involved in plant low temperature response. Gene ontology (GO) annotations based on similarities to database sequences were assigned to these marker sequences, and indicated potential involvement in signal transduction pathways in response to stress factors and epigenetic processes, as well as auxin transport mechanisms. Furthermore, relative gene expressions for three of six of new identified genes (Hv.ATPase, Hv.DDM1, and Hv.BIG) were assessed within four barley genotypes of different FT. A physiological assessment of FT was conducted based on plant survival rates in two field-laboratory and one laboratory experiments. The results suggested that plant survival rate after freezing but not the degree of freezing-induced leaf damage between the tested accessions can be correlated with the degree of low-temperature downregulation of the studied candidate genes, which encoded proteins involved in the control of plant growth and development. Additionally, candidate genes for qRT-PCR suitable for the analysis of cold acclimation response in barley were suggested after validation.
Author Anna Fiust (FoAE / DoPP)
Anna Fiust,,
- Department of Plant Physiology
, Marcin Rapacz (FoAaE / DoPP)
Marcin Rapacz,,
- Department of Plant Physiology
Journal seriesJournal of Plant Physiology, ISSN 0176-1617, e-ISSN 1618-1328, (N/A 100 pkt)
Issue year2020
Vol244
Pages1-10
Publication size in sheets0.5
Article number153049
Keywords in EnglishFreezing tolerance, Candidate gene, Barley
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science; 1314 Physiology
DOIDOI:10.1016/j.jplph.2019.153049
URL https://www.sciencedirect.com/science/article/pii/S0176161719301725/pdfft?md5=97109849cd36c8a180791ab15fa2e715&pid=1-s2.0-S0176161719301725-main.pdf
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.174; WoS Impact Factor: 2018 = 2.825 (2) - 2018=3.236 (5)
Citation count*
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FinansowanieThis work was supported by the National Science Centre, Poland [UMO-2013/09/N/NZ9/01588].
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