Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

Iwona Żur , Ewa Dubas , Aneta Słomka , Franciszek Dubert , Elżbieta Kuta , Agnieszka Płażek

Abstract

Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of the species. Therefore, the goal of this paper was to investigate the requirements of androgenesis in Miscanthus. The standard protocols used for monocotyledonous plants were applied with many modifications regarding the developmental stage of the explants at the time of culture initiation, stress treatment applied to panicles and isolated anthers as well as various chemical and physical parameters of in vitro culture conditions. Our results indicated that the induction of androgenesis in M. × giganteus is possible. However, the very low efficiency of the process and the lack of regeneration ability of the androgenic structures presently prevent the use of this technique.
Author Iwona Żur
Iwona Żur,,
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, Ewa Dubas
Ewa Dubas,,
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, Aneta Słomka
Aneta Słomka,,
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, Franciszek Dubert
Franciszek Dubert,,
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, Elżbieta Kuta
Elżbieta Kuta,,
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, Agnieszka Płażek (FoAE / DoPP)
Agnieszka Płażek,,
- Department of Plant Physiology
Journal seriesPlant Reproduction ISSN: 2194-7953, ISSN 2194-7953, (0 pkt)
Issue year2013
No26
Pages297-307
Publication size in sheets0.5
Keywords in EnglishAnther culture, Honey, Meiotic, abnormalities, Microspore embryogenesis
DOIDOI:10.1007/s00497-013-0219-6
URL http://link.springer.com/article/10.1007%2Fs00497-013-0219-6
Internal identifierWRE/2013/154
Languageen angielski
Score (nominal)0
Score sourcejournalList
Citation count*4 (2016-06-20)
Additional fields
FinansowanieMNiSW Nr 810/NCOST/2010/0 COST Action FA 0903
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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