Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels

Katarzyna Ropka-Molik , Klaudia Pawlina-Tyszko , Kacper Żukowski , Mirosław Tyra , Natalia Derebecka , Joanna Wesoły , Tomasz Szmatoła , Katarzyna Piórkowska

Abstract

Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs.
Author Katarzyna Ropka-Molik - The National Research Institute of Animal Production
Katarzyna Ropka-Molik,,
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, Klaudia Pawlina-Tyszko - The National Research Institute of Animal Production
Klaudia Pawlina-Tyszko,,
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, Kacper Żukowski - The National Research Institute of Animal Production
Kacper Żukowski,,
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, Mirosław Tyra - The National Research Institute of Animal Production
Mirosław Tyra,,
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, Natalia Derebecka - Adam Mickiewicz University (UAM)
Natalia Derebecka,,
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, Joanna Wesoły - Adam Mickiewicz University (UAM)
Joanna Wesoły,,
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, Tomasz Szmatoła (VET / CfEaIM) - The National Research Institute of Animal Production
Tomasz Szmatoła,,
- Center for Experimental and Innovative Medicine
, Katarzyna Piórkowska - The National Research Institute of Animal Production
Katarzyna Piórkowska,,
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Journal seriesGenes, ISSN 2073-4425, (N/A 100 pkt)
Issue year2020
Vol11
No6
Pages1-20
Publication size in sheets0.95
Article number600
Keywords in EnglishPig; fatness; obesity; extracellular matrix; fat deposition; lipid metabolism; NGS
ASJC Classification2716 Genetics(clinical); 1311 Genetics
DOIDOI:10.3390/genes11060600
URL https://www.mdpi.com/2073-4425/11/6/600/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels of 17-06-2020
867,03 KB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.864; WoS Impact Factor: 2018 = 3.331 (2) - 2018=3.484 (5)
Citation count*
Additional fields
FinansowanieThis research was funded by statutory activity of the National Research Institute of Animal Production, grant number 04.-0.14.1 (cost of biological material collection and storage) and grant number 01-18- 05-21 (cost of genetic analyses)
Oświadczenie o afiljacjiT. Szmatoła: załączone, afiljacja do UR
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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