Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels
Katarzyna Ropka-Molik , Klaudia Pawlina-Tyszko , Kacper Żukowski , Mirosław Tyra , Natalia Derebecka , Joanna Wesoły , Tomasz Szmatoła , Katarzyna Piórkowska
AbstractFat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs.
|Journal series||Genes, ISSN 2073-4425, (N/A 100 pkt)|
|Publication size in sheets||0.95|
|Keywords in English||Pig; fatness; obesity; extracellular matrix; fat deposition; lipid metabolism; NGS|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||: 2018 = 0.864; : 2018 = 3.331 (2) - 2018=3.484 (5)|
|Finansowanie||This research was funded by statutory activity of the National Research Institute of Animal Production, grant number 04.-0.14.1 (cost of biological material collection and storage) and grant number 01-18- 05-21 (cost of genetic analyses)|
|Oświadczenie o afiljacji||T. Szmatoła: załączone, afiljacja do UR|
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